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LearnPA5-26189
antibody from Invitrogen Antibodies
Targeting: CDC73
C1orf28, FIHP, HRPT1, HRPT2, parafibromin
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-26189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cdc73 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Local translation in nuclear condensate amyloid bodies.
Stress-specific aggregation of proteins in the amyloid bodies.
Theodoridis PR, Bokros M, Marijan D, Balukoff NC, Wang D, Kirk CC, Budine TD, Goldsmith HD, Wang M, Audas TE, Lee S
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)
Proceedings of the National Academy of Sciences of the United States of America 2021 Feb 16;118(7)
Stress-specific aggregation of proteins in the amyloid bodies.
Marijan D, Tse R, Elliott K, Chandhok S, Luo M, Lacroix E, Audas TE
FEBS letters 2019 Nov;593(22):3162-3172
FEBS letters 2019 Nov;593(22):3162-3172
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a CDC73 polyclonal antibody (Product # PA5-26189) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, fluor-conjugated secondary antibody (green). Actin filaments have been labeled with red dye conjugated phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cdc73 in paraformaldehyde-fixed, paraffin-embedded human adrenal gland tissue sections. Samples were incubated with Cdc73 polyclonal antibody (Product # PA5-26189) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cdc73 in paraformaldehyde-fixed, paraffin-embedded human adrenal gland tissue sections. Samples were incubated with Cdc73 polyclonal antibody (Product # PA5-26189) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MCF-7 cells using a CDC73 polyclonal antibody (Product # PA5-26189) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MCF-7 cells using a CDC73 polyclonal antibody (Product # PA5-26189) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
