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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- AM32639SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti ADAMTS1 / METH1
- Antibody type
- Monoclonal
- Antigen
- Myc-His carboxyl tagged full-length recombinant protein based on the Human sequence.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D4E11B6
- Vial size
- 0.1 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot Analysis: A431 cell lysate was probed with anti-ADAMTS-1 (1/4000). Proteins were visualized using a Goat anti-Mouse secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates ~65 kDa form of ADAMTS-1.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation: 1/50 dilution of clone 5C6D5 immunoprecipitated ADAMTS-1 from 500 µg of (1) HEK293 whole cell lysate or (2) PC-12 whole cell lysate on a representative lot, as confirmed by immunoblot using clone 5D4E11B6.
