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LearnPA5-29825
antibody from Invitrogen Antibodies
Targeting: MBNL1
EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-29825 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MBNL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, Jurkat.
- Concentration
- 0.45 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Muscleblind-like 1 using 30 µg of Jurkat lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Muscleblind-like 1 polyclonal antibody (Product # PA5-29825) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MBNL1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MBNL1 Polyclonal Antibody (Product # PA5-29825) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MBNL1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: MBNL1 Polyclonal Antibody (Product # PA5-29825) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
