Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA1-31351 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Golgi protein 58k Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX19072-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Golgi protein, 58k in human Liver lysate (35 µg protein in RIPA buffer) using a Golgi protein, 58k polyclonal antibody (Product # PA1-31351) at a dilution of 0.03 µg/mL following incubation for 1 hour and detected using chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Golgi protein 58k was performed by loading 35 µg (in RIPA buffer) of human liver lyssate. Proteins were transferred to a membrane and probed with a Golgi protein 58k Polyclonal Antibody (Product # PA1-31351) at a dilution of 0.05 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Golgi protein 58k Polyclonal Antibody (Product # PA1-31351) and a 58kDa band corresponding to Golgi protein 58k was observed in Hep G2, Mouse Liver and Rat Liver but was not observed in HeLa, A549, HEK-293, Mouse Kidney, Mouse Heart and Rat Brain which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), HEK-293 (Lane 4), Mouse Liver (Lane 5), Mouse Kidney (Lane 6), Mouse Heart (Lane 7), Rat Liver (Lane 8), Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Golgi protein 58k in human liver tissue using Golgi protein 58k Polyclonal Antibody (Product # PA1-31351) at a dilution of 3.8 µg/mL. Antigen retrieval : citrate buffer pH 6.