PA1-31351

antibody from Invitrogen Antibodies

Targeting: FTCD

Antibody data

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Validation data

Product number: PA1-31351 - Provider product page
Provider: Invitrogen Antibodies
Product name: Golgi protein 58k Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX19072-PEP)..
Concentration: 0.5 mg/mL

FTCD protein structure - PA1-31351 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Golgi protein, 58k in human Liver lysate (35 µg protein in RIPA buffer) using a Golgi protein, 58k polyclonal antibody (Product # PA1-31351) at a dilution of 0.03 µg/mL following incubation for 1 hour and detected using chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Golgi protein 58k was performed by loading 35 µg (in RIPA buffer) of human liver lyssate. Proteins were transferred to a membrane and probed with a Golgi protein 58k Polyclonal Antibody (Product # PA1-31351) at a dilution of 0.05 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Golgi protein 58k Polyclonal Antibody (Product # PA1-31351) and a 58kDa band corresponding to Golgi protein 58k was observed in Hep G2, Mouse Liver and Rat Liver but was not observed in HeLa, A549, HEK-293, Mouse Kidney, Mouse Heart and Rat Brain which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), HEK-293 (Lane 4), Mouse Liver (Lane 5), Mouse Kidney (Lane 6), Mouse Heart (Lane 7), Rat Liver (Lane 8), Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).