Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-1448 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1448, RRID:AB_2107000
- Product name
- Goat Polyclonal 58K Golgi Protein Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Variants (NP_006648.1
- Reactivity
- Human, Mouse, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome.
A novel 58-kDa protein associates with the Golgi apparatus and microtubules.
Hagiwara H, Tajika Y, Matsuzaki T, Suzuki T, Aoki T, Takata K
Histochemistry and cell biology 2006 Aug;126(2):251-9
Histochemistry and cell biology 2006 Aug;126(2):251-9
A novel 58-kDa protein associates with the Golgi apparatus and microtubules.
Bloom GS, Brashear TA
The Journal of biological chemistry 1989 Sep 25;264(27):16083-92
The Journal of biological chemistry 1989 Sep 25;264(27):16083-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Analysis (0.03ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NB100-1448] - Staining of paraffin embedded Human Liver. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.