PA5-22075

antibody from Invitrogen Antibodies

Targeting: FTCD

Antibody data

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Validation data

FTCD protein structure - PA5-22075 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Golgi protein 58k Polyclonal Antibody (Product # PA5-22075). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. Golgi protein 58k Polyclonal Antibody (Product # PA5-22075) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Golgi protein 58k Polyclonal Antibody (Product # PA5-22075). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. Golgi protein 58k Polyclonal Antibody (Product # PA5-22075) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Golgi protein 58k was achieved by transfecting Hep G2 with Golgi protein 58k specific siRNAs (Silencer® select Product # S21278, S21280). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Golgi protein 58k knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Golgi protein 58k Polyclonal Antibody (Product # PA5-22075, 1:1000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Golgi protein 58k.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Golgi protein 58k Polyclonal Antibody(Product # PA5-22075) and a 58kDa band corresponding to Golgi protein 58k was observed across all cell lines and tissues tested except HeLa, HEK-293, Mouse Kidney, Mouse Heart and Rat Brain which are reported to be negative. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3), Mouse Liver (Lane 4), Mouse Kidney (Lane 5), Mouse Heart (Lane 6), Rat Liver (Lane 7) and Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752).