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- Antigen structure
- References [2]
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- Product number
- PA5-34380 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCP110 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human colon tissue lysate. PA5-34380 can be used with blocking peptide PEP-1423.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Cell cycle-targeting microRNAs promote differentiation by enforcing cell-cycle exit.
miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110.
Otto T, Candido SV, Pilarz MS, Sicinska E, Bronson RT, Bowden M, Lachowicz IA, Mulry K, Fassl A, Han RC, Jecrois ES, Sicinski P
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 3;114(40):10660-10665
Proceedings of the National Academy of Sciences of the United States of America 2017 Oct 3;114(40):10660-10665
miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110.
Song R, Walentek P, Sponer N, Klimke A, Lee JS, Dixon G, Harland R, Wan Y, Lishko P, Lize M, Kessel M, He L
Nature 2014 Jun 5;510(7503):115-20
Nature 2014 Jun 5;510(7503):115-20
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 miR-34/449 miRNAs are required for ciliogenesis by repressing c p110 a. cp110 mRNA is derepressed in TKO tracheal epithelia (n=4). Paired t -test, ns P >0.05, * P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Figure 7 cp110 is a direct target of miR-34/449 miRNAs a. A schematic representation of two predicted miR-34/449 binding sites in the mouse c p110 3'UTR and in the luciferase reporter construct that contains cp110 3'UTR. b. Cp110 protein levels at postnatal day 23 are elevated in miR-34/449 TKO tracheal epithelia. c. The expression of Luc-cp110-3'UTR exhibits miR-34b- dependent repression in NIH/3T3 cells. Error bars represent s.e.m., n=3. Paired t -test, * P < 0.05. d. A schematic representation of one predicted miR-34/449 binding site in the frog c p110 3'UTR. A truncated cp110 construct, cp110 Delta3'UTR, was made to generate a cp110 cDNA without the miR-34/449 target site. e. Real time PCR monitoring cp110 reveals elevated mRNA expression levels of cp110 in miR-34/440 morphant frog skin explants as compared to Ctrl MO injected specimens. f. Timeline of MCC ciliation and recapitulation of ciliation defects in skin explants (animal caps). Representative confocal images from staged whole embryos and skin explants injected with either Ctrl MO or miR-34/449 MOs show the onset of ciliation at stage 26 and fully ciliated skin ectoderm at stage 32 in whole embryos and Ctrl MO injected skin explants. miR-34/449 MOs injected skin explants develop MCC ciliogenesis defects comparable to whole embryo treatment. Cilia: Ac-alpha-Tub (red), Actin: Phalloidin-488 (green) and nuclei: DAPI (blue). g. Expression of cp110 , foxj1 and miR-34/449 RNAs duri
