RQ4141

antibody from NSJ Bioreagents

Targeting: CCP110 CP110, KIAA0419

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4141

Provider

NSJ Bioreagents

Product name

CP110 Antibody / Centriolar coiled-coil protein of 110 kDa

Antibody type

Polyclonal

Description

This highly specific CP110 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the CP110 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) rat testis, 3) mouse testis, 4) mouse spleen and 5) mouse thymus lysate with CP110 antibody at 0.5ug/ml. Expected molecular weight ~110 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human MOLT4, 2) human HeLa, 3) human PANC-1, 4) rat PC-12 and 5) mouse RAW264.7 cell lysate with CP110 antibody. Expected molecular weight ~110 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HeLa cells with CP110 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse testis tissue with CP110 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat testis tissue with CP110 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with CP110 antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human testicular germ cell tumor tissue with CP110 antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colorectal adenocarcinoma tissue with CP110 antibody. Required HIER: steam section in pH8 EDTA buffer for 20 min and allow to cool prior to testing.