MA1-23284

antibody from Invitrogen Antibodies

Targeting: RPA2

Immunoprecipitation

Antibody data

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Validation data

Product number: MA1-23284 - Provider product page
Provider: Invitrogen Antibodies
Product name: RPA2 Monoclonal Antibody (12F3.3)
Antibody type: Monoclonal
Antigen: Other
Description: Recommended positive controls: MCF7, T24, Neuro2A, GL261, BCL-1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 12F3.3
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RPA2 protein structure - MA1-23284 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RPA32 in HeLa (IR treated) whole cell extract with no radiation (lane 1), 1hr (lane 2) 2hr (lane 3) and 4hr (lane 4) exposure using a RPA32 monoclonal antibody (Product # MA1-23284).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RPA32 Polyclonal Antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and blotted with RPA32 Polyclonal Antibody RPA2 Monoclonal Antibody (12F3.3) (Product # MA1-23284) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RPA2 was achieved by transfecting HeLa with RPA2 specific siRNAs (Silencer® select Product # s12132, s12130). Western blot analysis (Fig. a) was performed using whole cell extracts (1% SDS) from the RPA2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RPA2 Monoclonal Antibody (12F3.3) (Product # MA1-23284, 1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPA2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1%SDS) (30 µg lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), PC-12 (Lane 3), Hep G2 (Lane 4), MCF7 (Lane 5), Jurkat (Lane 6), Neuro-2A (Lane 7), HEK293T (Lane 8) and NIH/3T3 (Lane 9). The blot was probed with Anti-RPA2 Monoclonal Antibody (12F3.3) (Product # MA1-23284, 1 µg/mL dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to RPA2 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RPA32 Polyclonal Antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and blotted with RPA32 Polyclonal Antibody RPA2 Monoclonal Antibody (12F3.3) (Product # MA1-23284) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.