MA1-25889

antibody from Invitrogen Antibodies

Targeting: RPA2

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA1-25889

Provider

Invitrogen Antibodies

Product name

Anti-RPA2 Monoclonal Antibody (RPA34-20)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1-25896 detects MDM2 from human samples. MA1-25896 has been successfully used in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. Heat pretreatment is required for paraffin sections. The MA1-25896 immunogen is human MDM2 protein.

Reactivity

Human, Mouse, Yeast

Host

Mouse

Isotype

IgG

Antibody clone number

RPA34-20

Vial size

50 µg

Concentration

0.1 mg/ml

Storage

4° C, do not freeze

References

Submitted references

Dpb11 protein helps control assembly of the Cdc45·Mcm2-7·GINS replication fork helicase.

Dhingra N, Bruck I, Smith S, Ning B, Kaplan DL
The Journal of biological chemistry 2015 Mar 20;290(12):7586-601

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The replication initiation protein Sld2 regulates helicase assembly.

Bruck I, Kaplan DL
The Journal of biological chemistry 2014 Jan 24;289(4):1948-59

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Cdc45 protein-single-stranded DNA interaction is important for stalling the helicase during replication stress.

Bruck I, Kaplan DL
The Journal of biological chemistry 2013 Mar 15;288(11):7550-63

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RPA34 in methanol fixed cells using a RPA34 monoclonal antibody (Product # MA1-25889).