10412-1-Ap

antibody from Proteintech Group

Targeting: RPA2

Provider product page for 10412-1-Ap

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [28]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 10412-1-Ap - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10412-1-Ap, RRID:AB_2269665
Product name: RPA2 antibody
Antibody type: Polyclonal
Description: RPA2 antibody (Cat. #10412-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RPA2 protein structure - 10412-1-Ap shown in red.

Submitted references A circulating microRNA-based diagnostic model for breast cancer, in which the miR-139-3p/RPA2 axis regulates the sensitivity to DNA-damaging agents.

Liu J, Wang Y, Xu X, Zhang Y, Liu X, Liu X, Wang S, Zhao S, Zhang J
Translational cancer research 2026 Jan 31;15(1):60

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini-chromosome maintenance complex and causes DNA damage.

Tsuyama T, Takayama N, Tanaka R, Arai Y, Yamaguchi Y, Nawata Y, Azuma Y, Tada S
FEBS open bio 2026 Feb;16(2):352-364

LAP2α drives breast tumorigenesis by mitigating replication stress.

Ma Y, Qin Y, Bao P, Wei A, Yang Z, Liu L, Liu S, Foisner R, Shi L, Zhang Q, Bao K
Cell death & disease 2026 Feb 3;17(1):201

Phospho-TRIM21 orchestrates RPA2 ubiquitination switch to promote homologous recombination and tumor radio/chemo-resistance.

Zhang J, Chen B, Xu F, Wang R, Zhao X, Yao Z, Zhang J, Zhou S, Xu A, Wu L, Zhao G
Oncogene 2025 May;44(16):1106-1117

Nuclear-Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance.

Liu H, Feng J, Pan T, Zhang P, Ye L, Jiang Z, Zhou Z, Mao Q, Li J, Yang X, Gao P, Huang D, Zhang H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jun;12(22):e2416590

FOXD3 promotes homologous recombination repair and genomic stability by facilitating MRE11-mediated DNA end resection.

Xu S, Zhang J, Gao C, Xiong Z, Gong Y, Chai B, Chen H, Xu X
Acta biochimica et biophysica Sinica 2025 Jun 13;57(10):1601-1610

Targeting NAT10 attenuates homologous recombination via destabilizing DNA:RNA hybrids and overcomes PARP inhibitor resistance in cancers.

Xu Z, Zhu M, Geng L, Zhang J, Xia J, Wang Q, An H, Xia A, Yu Y, Liu S, Tong J, Zhu WG, Jiang Y, Sun B
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2025 Jul;81:101241

TTK activates ATR through RPA2 phosphorylation to promote olaparib resistance in ovarian cancer.

Qi G, Ma H, Chen J, Gai P, Teng K
Communications biology 2025 Jul 5;8(1):1011

DDX39A resolves replication fork-associated RNA-DNA hybrids to balance fork protection and cleavage for genomic stability maintenance.

Xu Z, Nie C, Liao J, Ma Y, Zhou XA, Li X, Li S, Lin H, Luo Y, Cheng K, Mao Z, Zhang L, Pan Y, Chen Y, Wang W, Wang J
Molecular cell 2025 Feb 6;85(3):490-505.e11

MRE11 is essential for the long-term viability of undifferentiated spermatogonia.

Tang Z, Liang Z, Zhang B, Xu X, Li P, Li L, Lu LY, Liu Y
Cell proliferation 2024 Sep;57(9):e13685

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors.

Wang Y, Liu X, Zuo X, Wang C, Zhang Z, Zhang H, Zeng T, Chen S, Liu M, Chen H, Song Q, Li Q, Yang C, Le Y, Xing J, Zhang H, An J, Jia W, Kang L, Zhang H, Xie H, Ye J, Wu T, He F, Zhang X, Li Y, Zhou G
Cell genomics 2024 May 8;4(5):100550

Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression.

Zhao Y, Liu W, Li Y, Ma J, Liu T, Cui H, Deng Y, Liao X, Wang Z
Journal of virology 2024 Mar 19;98(3):e0151523

Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer.

Ding JH, Xiao Y, Yang F, Song XQ, Xu Y, Ding XH, Ding R, Shao ZM, Di GH, Jiang YZ
Science translational medicine 2024 Jan 3;16(728):eadg7740

PAPAS promotes differentiation of mammary epithelial cells and suppresses breast carcinogenesis.

Ren S, Bai F, Schnell V, Stanko C, Ritsch M, Schenk T, Barth E, Marz M, Wang B, Pei XH, Bierhoff H
Cell reports 2024 Jan 23;43(1):113644

ADAR1 links R-loop homeostasis to ATR activation in replication stress response.

Zhang B, Li Y, Zhang J, Wang Y, Liang C, Lu T, Zhang C, Liu L, Qin Y, He J, Zhao X, Yu J, Hao J, Yang J, Li MJ, Yao Z, Ma S, Cheng H, Cheng T, Shi L
Nucleic acids research 2023 Nov 27;51(21):11668-11687

Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair.

Li G, Yang X, Luo X, Wu Z, Yang H
Cell & bioscience 2023 Nov 25;13(1):215

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice.

Jiang X, Cheng Y, Zhu Y, Xu C, Li Q, Xing X, Li W, Zou J, Meng L, Azhar M, Cao Y, Tong X, Qin W, Zhu X, Bao J
Nature communications 2023 Jun 22;14(1):3729

NKAP acts with HDAC3 to prevent R-loop associated genome instability.

Zhang X, Duan J, Li Y, Jin X, Wu C, Yang X, Lu W, Ge W
Cell death and differentiation 2023 Jul;30(7):1811-1828

Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin.

Zhang T, Yang H, Zhou Z, Bai Y, Wang J, Wang W
Nature communications 2022 Sep 1;13(1):5133

The ZFP541-KCTD19 complex is essential for pachytene progression by activating meiotic genes during mouse spermatogenesis.

Li Y, Meng R, Li S, Gu B, Xu X, Zhang H, Tan X, Shao T, Wang J, Xu D, Wang F
Journal of genetics and genomics = Yi chuan xue bao 2022 Nov;49(11):1029-1041

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Zhao J, Tian S, Guo Q, Bao K, Yu G, Wang X, Shen X, Zhang J, Chen J, Yang Y, Liu L, Li X, Hao J, Yang N, Liu Z, Ai D, Yang J, Zhu Y, Yao Z, Ma S, Zhang K, Shi L
Molecular cell 2022 Jul 21;82(14):2571-2587.e9

REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.

Chen Y, Jie X, Xing B, Wu Z, Yang X, Rao X, Xu Y, Zhou D, Dong X, Zhang T, Yang K, Li Z, Wu G
Cell death & disease 2022 Feb 3;13(2):110

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Gourdomichali O, Zonke K, Kattan FG, Makridakis M, Kontostathi G, Vlahou A, Doxakis E
Biology 2022 Feb 11;11(2)

Acquired temozolomide resistance in MGMT(low) gliomas is associated with regulation of homologous recombination repair by ROCK2.

Zhang X, Li T, Yang M, Du Q, Wang R, Fu B, Tan Y, Cao M, Chen Y, Wang Q, Hu R
Cell death & disease 2022 Feb 10;13(2):138

Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.

Ma S, Zhang J, Guo Q, Cao C, Bao K, Liu L, Chen CD, Liu Z, Yang J, Yang N, Yao Z, Shi L
Cancer letters 2022 Apr 1;530:29-44

C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.

Bai Y, Wang W, Li S, Zhan J, Li H, Zhao M, Zhou XA, Li S, Li X, Huo Y, Shen Q, Zhou M, Zhang H, Luo J, Sung P, Zhu WG, Xu X, Wang J
Molecular cell 2019 Sep 19;75(6):1299-1314.e6

A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair.

Li W, Wang HY, Zhao X, Duan H, Cheng B, Liu Y, Zhao M, Shu W, Mei Y, Wen Z, Tang M, Guo L, Li G, Chen Q, Liu X, Du HN
Science advances 2019 Mar;5(3):eaau7566

5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

Jiang W, Li YQ, Liu N, Sun Y, He QM, Jiang N, Xu YF, Chen L, Ma J
PloS one 2014;9(4):e93273

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A549 cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The RPA2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RPA2. This antibody recognizes human, mouse, rat antigen. The RPA2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 10412-1-Ap.pdf

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