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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- GTX83692 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83692, RRID:AB_10728074
- Product name
- RPA32 antibody [3A12]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPA2 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-RPA2 mouse monoclonal antibody (GTX83692) immunofluorescent staining of COS7 cells transiently transfected with RPA2
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83692, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-RPA2 mouse monoclonal antibody. (GTX83692, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-RPA2 antibody(GTX83692),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-RPA2 antibody(GTX83692),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY RPA2
