ABIN439699

antibody from antibodies-online

Targeting: RPA2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN439699

Provider

antibodies-online

Product name

anti-Replication Protein A2, 32kDa (RPA2) (pSer4), (pSer8) antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Epitope

pSer4, pSer8

Vial size

100 μL

Storage

Store at 4°C. Do not freeze.

Handling

Do not freeze.

References

Submitted references

Correction: Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells.

Qu J, Sun W, Zhong J, Lv H, Zhu M, Xu J, Jin N, Xie Z, Tan M, Lin SH, Geng M, Ding J, Huang M
The Journal of cell biology 2017 Jul 24;

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A subset of cancer cell lines is acutely sensitive to the Chk1 inhibitor MK-8776 as monotherapy due to CDK2 activation in S phase.

Sakurikar N, Thompson R, Montano R, Eastman A
Oncotarget 2016 Jan 12;7(2):1380-94

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The Mre11 nuclease is critical for the sensitivity of cells to Chk1 inhibition.

Thompson R, Montano R, Eastman A
PloS one 2012;7(8):e44021

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CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance but not initiation.

Kousholt AN, Fugger K, Hoffmann S, Larsen BD, Menzel T, Sartori AA, Sørensen CS
The Journal of cell biology 2012 Jun 25;197(7):869-76

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Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication.

Punchihewa C, Inoue A, Hishiki A, Fujikawa Y, Connelly M, Evison B, Shao Y, Heath R, Kuraoka I, Rodrigues P, Hashimoto H, Kawanishi M, Sato M, Yagi T, Fujii N
The Journal of biological chemistry 2012 Apr 20;287(17):14289-300

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Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase I-DNA complexes.

Regairaz M, Zhang YW, Fu H, Agama KK, Tata N, Agrawal S, Aladjem MI, Pommier Y
The Journal of cell biology 2011 Nov 28;195(5):739-49

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Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase δ.

Xu LH, Huang M, Fang SG, Liu DX
The Journal of biological chemistry 2011 Nov 11;286(45):39546-59

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RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response.

Liu S, Chu J, Yucer N, Leng M, Wang SY, Chen BP, Hittelman WN, Wang Y
The Journal of biological chemistry 2011 Jun 24;286(25):22314-22

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Alternative splicing of the human cytomegalovirus major immediate-early genes affects infectious-virus replication and control of cellular cyclin-dependent kinase.

Du G, Dutta N, Lashmit P, Stinski MF
Journal of virology 2011 Jan;85(2):804-17

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HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair.

Baldeyron C, Soria G, Roche D, Cook AJ, Almouzni G
The Journal of cell biology 2011 Apr 4;193(1):81-95

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The role of RPA2 phosphorylation in homologous recombination in response to replication arrest.

Shi W, Feng Z, Zhang J, Gonzalez-Suarez I, Vanderwaal RP, Wu X, Powell SN, Roti Roti JL, Gonzalo S, Zhang J
Carcinogenesis 2010 Jun;31(6):994-1002

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Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion.

Chen J, Luo Q, Yuan Y, Huang X, Cai W, Li C, Wei T, Zhang L, Yang M, Liu Q, Ye G, Dai X, Li B
Molecular and cellular biology 2010 Dec;30(24):5621-35

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Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.

Glauser DL, Seyffert M, Strasser R, Franchini M, Laimbacher AS, Dresch C, de Oliveira AP, Vogel R, Büning H, Salvetti A, Ackermann M, Fraefel C
Journal of virology 2010 Apr;84(8):3808-24

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Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.

Stephan H, Concannon C, Kremmer E, Carty MP, Nasheuer HP
Nucleic acids research 2009 Oct;37(18):6028-41

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Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.

Carson CT, Orazio NI, Lee DV, Suh J, Bekker-Jensen S, Araujo FD, Lakdawala SS, Lilley CE, Bartek J, Lukas J, Weitzman MD
The EMBO journal 2009 Mar 18;28(6):652-62

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Overall Cdk activity modulates the DNA damage response in mammalian cells.

Cerqueira A, Santamaría D, Martínez-Pastor B, Cuadrado M, Fernández-Capetillo O, Barbacid M
The Journal of cell biology 2009 Dec 14;187(6):773-80

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Human CtIP mediates cell cycle control of DNA end resection and double strand break repair.

Huertas P, Jackson SP
The Journal of biological chemistry 2009 Apr 3;284(14):9558-65

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DNA helicases Sgs1 and BLM promote DNA double-strand break resection.

Gravel S, Chapman JR, Magill C, Jackson SP
Genes & development 2008 Oct 15;22(20):2767-72

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BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways.

Kranz D, Dohmesen C, Dobbelstein M
The Journal of cell biology 2008 Jul 14;182(1):197-213

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Recombinant adeno-associated viral vectors are deficient in provoking a DNA damage response.

Fragkos M, Breuleux M, Clément N, Beard P
Journal of virology 2008 Aug;82(15):7379-87

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A novel cell response triggered by interphase centromere structural instability.

Morency E, Sabra M, Catez F, Texier P, Lomonte P
The Journal of cell biology 2007 Jun 4;177(5):757-68

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NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.

Manthey KC, Opiyo S, Glanzer JG, Dimitrova D, Elliott J, Oakley GG
Journal of cell science 2007 Dec 1;120(Pt 23):4221-9

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RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint.

Olson E, Nievera CJ, Klimovich V, Fanning E, Wu X
The Journal of biological chemistry 2006 Dec 22;281(51):39517-33

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UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK.

Cruet-Hennequart S, Coyne S, Glynn MT, Oakley GG, Carty MP
DNA repair 2006 Apr 8;5(4):491-504

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Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells.

Wu X, Shell SM, Zou Y
Oncogene 2005 Jul 7;24(29):4728-35

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Replication protein A (RPA) phosphorylation prevents RPA association with replication centers.

Vassin VM, Wold MS, Borowiec JA
Molecular and cellular biology 2004 Mar;24(5):1930-43

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC