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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-110204 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IRHAEKAPTN IVYKIDDMTA APMDVRQWVD TDDTSSENTV VPPETYVKVA GHLRSFQNKK SLVAFKIMPL EDMNEFTTHI LEVINAHMVL SKANSQPSAG RAPISNPGMS EAGNFGGNSF MPANGLTVAQ NQVLNLIKAC PRPEGLNFQD LKNQLKHMSV SSIKQAVDFL SNEGHIYSTV DDDHFKSTDA E
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.48 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of RPA2 in U-2OS cells. Samples were incubated in polyclonal RPA2 antibody (Product # PA5-110204) using a dilution of 1:100, followed by DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RPA2 in 200 μg extracts of NIH/3T3 cells. Samples were precipitated with 3 μg RPA2 Polyclonal antibody (Product # PA5-110204). Western blot was performed from the immunoprecipitate using RPA2 Polyclonal antibody (Product # PA5-110204) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RPA2 in paraffin-embedded human kidney cancer. Samples were incubated with RPA2 Polyclonal antibody (Product # PA5-110204) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of RPA2 in extracts of mouse spleen cells. Samples were incubated with RPA2 Polyclonal antibody (Product # PA5-110204) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of RPA2 in Jurkat cells. Samples were incubated in polyclonal RPA2 antibody (Product # PA5-110204) using a dilution of 1:1000.
