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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- A300-245A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-RPA32 (Ser4, Ser8) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 µg) from BeWo and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (Product # A300-245A lot 9) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6). For blotting immunoprecipitated Phospho RPA32 (S4/S8), affinity purified rabbit anti-RPA32 antibody A300-244A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody A300-245A (lot A300-245A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 µg/ml with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right). Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot 4) used at a dilution of 1:1,000 (1 µg/mL). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100. Counterstain: Alexa Fluor® 488 phalloidin (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human breast carcinoma, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (Product # A300-245A; lot 9) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse gut, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) (Product # A300-245A lot 9) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) (Cat. No. A300-245A Lot6) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with or without etoposide. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody (Product # A300-245A lot 9) used for IP at 6 µg per reaction followed by treatment with Lambda phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho RPA32 (S4/S8) (Product # A300-245A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPA32 antibody (Product # A300-244A).
