Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- NB100-544 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-544, RRID:AB_10002227
- Product name
- Rabbit Polyclonal RPA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-544 is specific for human RPA-32 pSer33 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references TRIP13 regulates DNA repair pathway choice through REV7 conformational change.
High speed of fork progression induces DNA replication stress and genomic instability.
Active DNA end processing in micronuclei of ovarian cancer cells.
53BP1 Mediates ATR-Chk1 Signaling and Protects Replication Forks under Conditions of Replication Stress.
ETAA1 acts at stalled replication forks to maintain genome integrity.
G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells.
Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation.
PC4 promotes genome stability and DNA repair through binding of ssDNA at DNA damage sites.
TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity.
Loss of Smu1 function de-represses DNA replication and over-activates ATR-dependent replication checkpoint.
A dual role of BRCA1 in two distinct homologous recombination mediated repair in response to replication arrest.
Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death.
Divergent S phase checkpoint activation arising from prereplicative complex deficiency controls cell survival.
Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis.
Protein phosphatase 2A-dependent dephosphorylation of replication protein A is required for the repair of DNA breaks induced by replication stress.
Clairmont CS, Sarangi P, Ponnienselvan K, Galli LD, Csete I, Moreau L, Adelmant G, Chowdhury D, Marto JA, D'Andrea AD
Nature cell biology 2020 Jan;22(1):87-96
Nature cell biology 2020 Jan;22(1):87-96
High speed of fork progression induces DNA replication stress and genomic instability.
Maya-Mendoza A, Moudry P, Merchut-Maya JM, Lee M, Strauss R, Bartek J
Nature 2018 Jul;559(7713):279-284
Nature 2018 Jul;559(7713):279-284
Active DNA end processing in micronuclei of ovarian cancer cells.
Tang Z, Yang J, Wang X, Zeng M, Wang J, Wang A, Zhao M, Guo L, Liu C, Li D, Chen J
BMC cancer 2018 Apr 16;18(1):426
BMC cancer 2018 Apr 16;18(1):426
53BP1 Mediates ATR-Chk1 Signaling and Protects Replication Forks under Conditions of Replication Stress.
Her J, Ray C, Altshuler J, Zheng H, Bunting SF
Molecular and cellular biology 2018 Apr 15;38(8)
Molecular and cellular biology 2018 Apr 15;38(8)
ETAA1 acts at stalled replication forks to maintain genome integrity.
Bass TE, Luzwick JW, Kavanaugh G, Carroll C, Dungrawala H, Glick GG, Feldkamp MD, Putney R, Chazin WJ, Cortez D
Nature cell biology 2016 Nov;18(11):1185-1195
Nature cell biology 2016 Nov;18(11):1185-1195
G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells.
Hasegawa D, Okabe S, Okamoto K, Nakano I, Shin-ya K, Seimiya H
Biochemical and biophysical research communications 2016 Feb 26;471(1):75-81
Biochemical and biophysical research communications 2016 Feb 26;471(1):75-81
Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation.
Yu ZC, Huang YF, Shieh SY
Nucleic acids research 2016 Feb 18;44(3):1133-50
Nucleic acids research 2016 Feb 18;44(3):1133-50
PC4 promotes genome stability and DNA repair through binding of ssDNA at DNA damage sites.
Mortusewicz O, Evers B, Helleday T
Oncogene 2016 Feb 11;35(6):761-70
Oncogene 2016 Feb 11;35(6):761-70
TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity.
Moudry P, Watanabe K, Wolanin KM, Bartkova J, Wassing IE, Watanabe S, Strauss R, Troelsgaard Pedersen R, Oestergaard VH, Lisby M, Andújar-Sánchez M, Maya-Mendoza A, Esashi F, Lukas J, Bartek J
The Journal of cell biology 2016 Feb 1;212(3):281-8
The Journal of cell biology 2016 Feb 1;212(3):281-8
Loss of Smu1 function de-represses DNA replication and over-activates ATR-dependent replication checkpoint.
Ren L, Liu Y, Guo L, Wang H, Ma L, Zeng M, Shao X, Yang C, Tang Y, Wang L, Liu C, Li M
Biochemical and biophysical research communications 2013 Jun 28;436(2):192-8
Biochemical and biophysical research communications 2013 Jun 28;436(2):192-8
A dual role of BRCA1 in two distinct homologous recombination mediated repair in response to replication arrest.
Feng Z, Zhang J
Nucleic acids research 2012 Jan;40(2):726-38
Nucleic acids research 2012 Jan;40(2):726-38
Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death.
Luo Y, Chen AY, Qiu J
Journal of virology 2011 Jan;85(1):133-45
Journal of virology 2011 Jan;85(1):133-45
Divergent S phase checkpoint activation arising from prereplicative complex deficiency controls cell survival.
Lau E, Chiang GG, Abraham RT, Jiang W
Molecular biology of the cell 2009 Sep;20(17):3953-64
Molecular biology of the cell 2009 Sep;20(17):3953-64
Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis.
Smith KD, Fu MA, Brown EJ
The Journal of cell biology 2009 Oct 5;187(1):15-23
The Journal of cell biology 2009 Oct 5;187(1):15-23
Protein phosphatase 2A-dependent dephosphorylation of replication protein A is required for the repair of DNA breaks induced by replication stress.
Feng J, Wakeman T, Yong S, Wu X, Kornbluth S, Wang XF
Molecular and cellular biology 2009 Nov;29(21):5696-709
Molecular and cellular biology 2009 Nov;29(21):5696-709
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Detection of Human phospho RPA32 (S33) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-phospho RPA32-S33 antibody NB100-544 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows Westen Blotting for total RPA32 using affinity purified rabbit anti-RPA32 antibody at 0.1 ug/ml with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit antiphospho RPA32 (S33) antibody used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.