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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab125728 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- RPA32/RPA2 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti human RPA32/RPA2 Antibody, Monoclonal (1591CT183.68.6), could be used for WB, IHC and so on.Application:WB: 1/4000 IHC_1/100~1/500Protein Function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-270- Antibody clone number
- 1591CT183.68.6
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- RPA32/RPA2 Mouse mAb (Ab125728) - Western Blot All lanes: RPA32/RPA2 Mouse mAb (Ab125728) at 1/4000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 31 kDa Exposure time: 50.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- RPA32/RPA2 Mouse mAb (Ab125728) - IHC RPA32/RPA2 Mouse mAb (Ab125728) staining RFA2 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
