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- Western blot [3]
- Immunohistochemistry [4]
- Flow cytometry [2]
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- Product number
- NBP2-02182 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal RPA2 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references LIM Protein Ajuba Participates in the Repression of the ATR-Mediated DNA Damage Response.
Kalan S, Matveyenko A, Loayza D
Frontiers in genetics 2013;4:95
Frontiers in genetics 2013;4:95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
