Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-332 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-332, RRID:AB_10003085
- Product name
- Rabbit Polyclonal RPA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Human papillomaviruses recruit cellular DNA repair and homologous recombination factors to viral replication centers.
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart.
RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability.
RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling.
Gillespie KA, Mehta KP, Laimins LA, Moody CA
Journal of virology 2012 Sep;86(17):9520-6
Journal of virology 2012 Sep;86(17):9520-6
Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
Boichuk S, Hu L, Hein J, Gjoerup OV
Journal of virology 2010 Aug;84(16):8007-20
Journal of virology 2010 Aug;84(16):8007-20
The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart.
Ciccia A, Bredemeyer AL, Sowa ME, Terret ME, Jallepalli PV, Harper JW, Elledge SJ
Genes & development 2009 Oct 15;23(20):2415-25
Genes & development 2009 Oct 15;23(20):2415-25
RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability.
Xu D, Guo R, Sobeck A, Bachrati CZ, Yang J, Enomoto T, Brown GW, Hoatlin ME, Hickson ID, Wang W
Genes & development 2008 Oct 15;22(20):2843-55
Genes & development 2008 Oct 15;22(20):2843-55
RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling.
Stewart GS, Stankovic T, Byrd PJ, Wechsler T, Miller ES, Huissoon A, Drayson MT, West SC, Elledge SJ, Taylor AM
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 23;104(43):16910-5
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 23;104(43):16910-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody [NB100-332] - Whole cell lysate from 293T cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody [NB100-332] - Detection of Human and Mouse RPA32 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody [NB100-332] - Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RPA2 Antibody [NB100-332] - Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody NB100-332 used for IP at 3 ug per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (NB100-23017). For blotting immunoprecipitated Phospho RPA32 (S4/S8), NB100-23017 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB