Antibody data
- Antibody Data
- Antigen structure
- References [31]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [8]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-23017 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-23017, RRID:AB_1726226
- Product name
- Rabbit Polyclonal RPA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cancer cell stemness, responses to experimental genotoxic treatments, cytomegalovirus protein expression and DNA replication stress in pediatric medulloblastomas.
Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe.
H4K20me2 distinguishes pre-replicative from post-replicative chromatin to appropriately direct DNA repair pathway choice by 53BP1-RIF1-MAD2L2.
Correction: Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells.
Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells.
A subset of cancer cell lines is acutely sensitive to the Chk1 inhibitor MK-8776 as monotherapy due to CDK2 activation in S phase.
The Mre11 nuclease is critical for the sensitivity of cells to Chk1 inhibition.
CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance but not initiation.
Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication.
Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase I-DNA complexes.
Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase δ.
RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response.
Alternative splicing of the human cytomegalovirus major immediate-early genes affects infectious-virus replication and control of cellular cyclin-dependent kinase.
HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair.
The role of RPA2 phosphorylation in homologous recombination in response to replication arrest.
Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion.
Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.
Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.
Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.
Overall Cdk activity modulates the DNA damage response in mammalian cells.
Human CtIP mediates cell cycle control of DNA end resection and double strand break repair.
DNA helicases Sgs1 and BLM promote DNA double-strand break resection.
BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways.
Recombinant adeno-associated viral vectors are deficient in provoking a DNA damage response.
A novel cell response triggered by interphase centromere structural instability.
NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint.
UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK.
Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells.
Replication protein A (RPA) phosphorylation prevents RPA association with replication centers.
Replication protein A (RPA) phosphorylation prevents RPA association with replication centers.
Bartek J Jr, Merchut-Maya JM, Maya-Mendoza A, Fornara O, Rahbar A, Bröchner CB, Sehested A, Söderberg-Nauclér C, Bartek J, Bartkova J
Cell cycle (Georgetown, Tex.) 2020 Apr;19(7):727-741
Cell cycle (Georgetown, Tex.) 2020 Apr;19(7):727-741
Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe.
Warren NJH, Eastman A
The Journal of biological chemistry 2019 Feb 8;294(6):1763-1778
The Journal of biological chemistry 2019 Feb 8;294(6):1763-1778
H4K20me2 distinguishes pre-replicative from post-replicative chromatin to appropriately direct DNA repair pathway choice by 53BP1-RIF1-MAD2L2.
Simonetta M, de Krijger I, Serrat J, Moatti N, Fortunato D, Hoekman L, Bleijerveld OB, Altelaar AFM, Jacobs JJL
Cell cycle (Georgetown, Tex.) 2018;17(1):124-136
Cell cycle (Georgetown, Tex.) 2018;17(1):124-136
Correction: Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells.
Qu J, Sun W, Zhong J, Lv H, Zhu M, Xu J, Jin N, Xie Z, Tan M, Lin SH, Geng M, Ding J, Huang M
The Journal of cell biology 2017 Jul 24;216(8):2597-8
The Journal of cell biology 2017 Jul 24;216(8):2597-8
Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells.
Qu J, Sun W, Zhong J, Lv H, Zhu M, Xu J, Jin N, Xie Z, Tan M, Lin SH, Geng M, Ding J, Huang M
The Journal of cell biology 2017 Feb;216(2):409-424
The Journal of cell biology 2017 Feb;216(2):409-424
A subset of cancer cell lines is acutely sensitive to the Chk1 inhibitor MK-8776 as monotherapy due to CDK2 activation in S phase.
Sakurikar N, Thompson R, Montano R, Eastman A
Oncotarget 2016 Jan 12;7(2):1380-94
Oncotarget 2016 Jan 12;7(2):1380-94
The Mre11 nuclease is critical for the sensitivity of cells to Chk1 inhibition.
Thompson R, Montano R, Eastman A
PloS one 2012;7(8):e44021
PloS one 2012;7(8):e44021
CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance but not initiation.
Kousholt AN, Fugger K, Hoffmann S, Larsen BD, Menzel T, Sartori AA, Sørensen CS
The Journal of cell biology 2012 Jun 25;197(7):869-76
The Journal of cell biology 2012 Jun 25;197(7):869-76
Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication.
Punchihewa C, Inoue A, Hishiki A, Fujikawa Y, Connelly M, Evison B, Shao Y, Heath R, Kuraoka I, Rodrigues P, Hashimoto H, Kawanishi M, Sato M, Yagi T, Fujii N
The Journal of biological chemistry 2012 Apr 20;287(17):14289-300
The Journal of biological chemistry 2012 Apr 20;287(17):14289-300
Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase I-DNA complexes.
Regairaz M, Zhang YW, Fu H, Agama KK, Tata N, Agrawal S, Aladjem MI, Pommier Y
The Journal of cell biology 2011 Nov 28;195(5):739-49
The Journal of cell biology 2011 Nov 28;195(5):739-49
Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase δ.
Xu LH, Huang M, Fang SG, Liu DX
The Journal of biological chemistry 2011 Nov 11;286(45):39546-59
The Journal of biological chemistry 2011 Nov 11;286(45):39546-59
RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response.
Liu S, Chu J, Yucer N, Leng M, Wang SY, Chen BP, Hittelman WN, Wang Y
The Journal of biological chemistry 2011 Jun 24;286(25):22314-22
The Journal of biological chemistry 2011 Jun 24;286(25):22314-22
Alternative splicing of the human cytomegalovirus major immediate-early genes affects infectious-virus replication and control of cellular cyclin-dependent kinase.
Du G, Dutta N, Lashmit P, Stinski MF
Journal of virology 2011 Jan;85(2):804-17
Journal of virology 2011 Jan;85(2):804-17
HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair.
Baldeyron C, Soria G, Roche D, Cook AJ, Almouzni G
The Journal of cell biology 2011 Apr 4;193(1):81-95
The Journal of cell biology 2011 Apr 4;193(1):81-95
The role of RPA2 phosphorylation in homologous recombination in response to replication arrest.
Shi W, Feng Z, Zhang J, Gonzalez-Suarez I, Vanderwaal RP, Wu X, Powell SN, Roti Roti JL, Gonzalo S, Zhang J
Carcinogenesis 2010 Jun;31(6):994-1002
Carcinogenesis 2010 Jun;31(6):994-1002
Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion.
Chen J, Luo Q, Yuan Y, Huang X, Cai W, Li C, Wei T, Zhang L, Yang M, Liu Q, Ye G, Dai X, Li B
Molecular and cellular biology 2010 Dec;30(24):5621-35
Molecular and cellular biology 2010 Dec;30(24):5621-35
Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.
Glauser DL, Seyffert M, Strasser R, Franchini M, Laimbacher AS, Dresch C, de Oliveira AP, Vogel R, Büning H, Salvetti A, Ackermann M, Fraefel C
Journal of virology 2010 Apr;84(8):3808-24
Journal of virology 2010 Apr;84(8):3808-24
Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.
Stephan H, Concannon C, Kremmer E, Carty MP, Nasheuer HP
Nucleic acids research 2009 Oct;37(18):6028-41
Nucleic acids research 2009 Oct;37(18):6028-41
Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection.
Carson CT, Orazio NI, Lee DV, Suh J, Bekker-Jensen S, Araujo FD, Lakdawala SS, Lilley CE, Bartek J, Lukas J, Weitzman MD
The EMBO journal 2009 Mar 18;28(6):652-62
The EMBO journal 2009 Mar 18;28(6):652-62
Overall Cdk activity modulates the DNA damage response in mammalian cells.
Cerqueira A, Santamaría D, Martínez-Pastor B, Cuadrado M, Fernández-Capetillo O, Barbacid M
The Journal of cell biology 2009 Dec 14;187(6):773-80
The Journal of cell biology 2009 Dec 14;187(6):773-80
Human CtIP mediates cell cycle control of DNA end resection and double strand break repair.
Huertas P, Jackson SP
The Journal of biological chemistry 2009 Apr 3;284(14):9558-65
The Journal of biological chemistry 2009 Apr 3;284(14):9558-65
DNA helicases Sgs1 and BLM promote DNA double-strand break resection.
Gravel S, Chapman JR, Magill C, Jackson SP
Genes & development 2008 Oct 15;22(20):2767-72
Genes & development 2008 Oct 15;22(20):2767-72
BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways.
Kranz D, Dohmesen C, Dobbelstein M
The Journal of cell biology 2008 Jul 14;182(1):197-213
The Journal of cell biology 2008 Jul 14;182(1):197-213
Recombinant adeno-associated viral vectors are deficient in provoking a DNA damage response.
Fragkos M, Breuleux M, Clément N, Beard P
Journal of virology 2008 Aug;82(15):7379-87
Journal of virology 2008 Aug;82(15):7379-87
A novel cell response triggered by interphase centromere structural instability.
Morency E, Sabra M, Catez F, Texier P, Lomonte P
The Journal of cell biology 2007 Jun 4;177(5):757-68
The Journal of cell biology 2007 Jun 4;177(5):757-68
NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
Manthey KC, Opiyo S, Glanzer JG, Dimitrova D, Elliott J, Oakley GG
Journal of cell science 2007 Dec 1;120(Pt 23):4221-9
Journal of cell science 2007 Dec 1;120(Pt 23):4221-9
RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint.
Olson E, Nievera CJ, Klimovich V, Fanning E, Wu X
The Journal of biological chemistry 2006 Dec 22;281(51):39517-33
The Journal of biological chemistry 2006 Dec 22;281(51):39517-33
UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK.
Cruet-Hennequart S, Coyne S, Glynn MT, Oakley GG, Carty MP
DNA repair 2006 Apr 8;5(4):491-504
DNA repair 2006 Apr 8;5(4):491-504
Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells.
Wu X, Shell SM, Zou Y
Oncogene 2005 Jul 7;24(29):4728-35
Oncogene 2005 Jul 7;24(29):4728-35
Replication protein A (RPA) phosphorylation prevents RPA association with replication centers.
Vassin VM, Wold MS, Borowiec JA
Molecular and cellular biology 2004 Mar;24(5):1930-43
Molecular and cellular biology 2004 Mar;24(5):1930-43
Replication protein A (RPA) phosphorylation prevents RPA association with replication centers.
Vassin VM, Wold MS, Borowiec JA
Molecular and cellular biology 2004 Mar;24(5):1930-43
Molecular and cellular biology 2004 Mar;24(5):1930-43
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with EPE (+) or mock treated (-). In B, immunoprecipitated RPA32 was either treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 lot 2 at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 ug/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds for phospho RPA32 Ser4/Ser8 (A and B) and 1 second for total RPA32 (A and B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified rabbit anti-RPA32 antibody A300-244A at 0.1 ug/ml with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of Human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 used at 0.1 ug/ml. Lower panel: Rabbit anti-RPA32 antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with EPE (+) and/or Lambda PP (+) or mock treated (-). Antibodies: RPA32 (S4/S8) was immunoprecipitated using affinity purified rabbit anti-RPA32 antibody NB100-332. For blotting immunoprecipitated Phospho RPA32 (S4/S8), rabbit anti-Phospho RPA32 (S4/S8) antibody NBP1-23017 was used at 0.1 ug/ml. Lower Panel: Rabbit anti-RPA antibody NB100-332. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- Phospho (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) used at a dilution of 1:250
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Mouse colon carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Section of human lung cancer. Antibody: Affinity purified rabbit antiPhospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Sample: FFPE section of mouse colon carcinoma CT26. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB