Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-158 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-158, RRID:AB_2180491
- Product name
- Mouse Monoclonal RPA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references 53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function.
Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA.
XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells.
Sundaravinayagam D, Rahjouei A, Andreani M, Tupiņa D, Balasubramanian S, Saha T, Delgado-Benito V, Coralluzzo V, Daumke O, Di Virgilio M
Cell reports 2019 Aug 6;28(6):1389-1399.e6
Cell reports 2019 Aug 6;28(6):1389-1399.e6
Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA.
Salas TR, Petruseva I, Lavrik O, Saintomé C
Nucleic acids research 2009 Jan;37(1):38-46
Nucleic acids research 2009 Jan;37(1):38-46
XRCC3 deficiency results in a defect in recombination and increased endoreduplication in human cells.
Yoshihara T, Ishida M, Kinomura A, Katsura M, Tsuruga T, Tashiro S, Asahara T, Miyagawa K
The EMBO journal 2004 Feb 11;23(3):670-80
The EMBO journal 2004 Feb 11;23(3):670-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.