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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF6654 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-RPA2 (T21) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human RPA2 when phosphorylated at T21.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P15927- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Phospho-RPA2 (T21) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour. PVDF Membrane was probed with 0.5 µg/mL of Human Phospho-RPA2 (T21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6654) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RPA2 (T21) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
