MA1-83191

antibody from Invitrogen Antibodies

Targeting: SLC2A4 GLUT4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-83191

Provider

Invitrogen Antibodies

Product name

GLUT4 Monoclonal Antibody (1F8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody does not react with dog. This product requires enzyme-mediated antigen retrieval prior to staining of paraffin sections. Proteinase K is recommended. Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al.

Reactivity

Human, Mouse, Rat, Porcine, Rabbit

Host

Mouse

Isotype

IgG

Antibody clone number

1F8

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-α pathway.

Zhang F, Liu L, Xie Y, Wang J, Chen X, Zheng S, Li Y, Dang Y
Open medicine (Warsaw, Poland) 2022;17(1):365-374

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Impaired HMG-CoA Reductase Activity Caused by Genetic Variants or Statin Exposure: Impact on Human Adipose Tissue, β-Cells and Metabolome.

Sarsenbayeva A, Jui BN, Fanni G, Barbosa P, Ahmed F, Kristófi R, Cen J, Chowdhury A, Skrtic S, Bergsten P, Fall T, Eriksson JW, Pereira MJ
Metabolites 2021 Aug 25;11(9)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 3T3-L1 lysate probed with a Glucose Transporter 4 monoclonal antibody (Product # MA1-83191) followed by Goat anti mouse IgG, Dylight 800 conjugate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of 3T3-L1 lysate probed with a Glucose Transporter 4 monoclonal antibody (Product # MA1-83191)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), tissue extracts of Mouse heart (Lane 7), Mouse Skin (Lane 8) and Mouse Skeletal Muscle (Lane 9). The blot was probed with Anti-GLUT4 Monoclonal Antibody (1F8) (Product # MA1-83191, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 53 kDa band corresponding to GLUT4 was observed across cell lines and tissue extracts tested and was also enhanced in 3T3-L1 after differentiating it to adipocytes. A 25 kDa band corresponding to free IgG was also observed in the tissue extracts.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Effects of simvastatin and simvastatin acid on adipocyte differentiation. ( A ) Representative images of differentiation rate of adipocytes treated without (Control) or with Simvastatin 100 nM (Sim 100 nM), simvastatin hydroxy acid 8 and 30 nM (SA 8 nM and SA 30 nM, respectively). Left to right: Nuclei are shown in blue (DAPI), lipid droplets are shown in green (Bodipy), brightfield image, and merged. ( B ) Quantification of adipocyte differentiation rate. ( C ) The differentiation rate was also assessed by measuring the mRNA expression of the master regulator of adipogenesis PPARG . GUSB was used as the housekeeping gene. Relative gene expression was measured as 2 -DeltaDeltaCt . ( D ) Representative blot of GLUT4 expression in adipocytes treated without (Control) or with Simvastatin 100 nM (Sim 100 nM), simvastatin hydroxy acid 30 nM and 8.0 nM (SA 30 nM and SA 8 nM), respectively, at day 14. ( E ) Quantification of GLUT4 expression. Graphs show mean +- S.E.M. of n = 3 independent experiments.