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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-17176 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT4 Monoclonal Antibody (3G10A3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17176 targets SLC2A4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples. The MA5-17176 immunogen is purified recombinant fragment of human SLC2A4 (amino acids: 224-353 ) expressed in E. Coli. MA5-17176 detects SLC2A4 which has a predicted molecular weight of approximately 54.8kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G10A3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes.
Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling.
Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation.
Increased glucose metabolism in Arid5b(-/-) skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1).
Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training.
Montt-Guevara MM, Finiguerra M, Marzi I, Fidecicchi T, Ferrari A, Genazzani AD, Simoncini T
Frontiers in endocrinology 2021;12:660815
Frontiers in endocrinology 2021;12:660815
Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling.
Bruno NE, Nwachukwu JC, Srinivasan S, Nettles CC, Izard T, Jin Z, Nowak J, Cameron MD, Boregowda SV, Phinney DG, Elemento O, Liu X, Ortlund EA, Houtman R, Stavreva DA, Hager GL, Kamenecka TM, Kojetin DJ, Nettles KW
Nature chemical biology 2021 Mar;17(3):307-316
Nature chemical biology 2021 Mar;17(3):307-316
Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation.
Slaughter VL, Rumsey JW, Boone R, Malik D, Cai Y, Sriram NN, Long CJ, McAleer CW, Lambert S, Shuler ML, Hickman JJ
Scientific reports 2021 Jun 23;11(1):13159
Scientific reports 2021 Jun 23;11(1):13159
Increased glucose metabolism in Arid5b(-/-) skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1).
Okazaki Y, Murray J, Ehsani A, Clark J, Whitson RH, Hirose L, Yanaka N, Itakura K
Biological research 2020 Oct 6;53(1):45
Biological research 2020 Oct 6;53(1):45
Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training.
Fernández-Verdejo R, Vanwynsberghe AM, Essaghir A, Demoulin JB, Hai T, Deldicque L, Francaux M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Feb;31(2):840-851
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Feb;31(2):840-851
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using SLC2A4 monoclonal antibody (Product # MA5-17176) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using SLC2A4 monoclonal antibody (Product # MA5-17176) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using SLC2A4 monoclonal antibody (Product # MA5-17176) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 D-Chiro-Ins does not modulate the expression of GLUT4. Mature SGBS adipocytes cells were treated with D-Chiro-Ins 10 -8 M, E2 10 -9 M or INS 10 -7 M, and combinations for 24 h. Cell lysates were immunoblotted with an anti-GLUT4 antibody, anti-p-GLUT4 antibody, and anti-GAPDH antibody. (A) Panels show representative blot of GLUT4, p-GLUT4 S488 and GAPDH. (B-D) Analysis of the relative expression level of GLUT4, p-GLUT4 and the ratio p-GLUT4/GLUT4; expression that was normalized with GAPDH and relative to the control. Values are presented as mean +- SEM of three independent experiments. Results were analyzed statistically by one-way RM ANOVA followed by Dunnett''s multiple comparisons test (*p < 0.05, **p < 0.001 versus control). D-Chiro-Ins, D-chiro-Inositol; E2, 17beta-estradiol, INS, insulin; GLUT4, Glucose Transporter 4; p-GLUT4, phospho-GLUT4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
