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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-32062 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RFESD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, MCF-7, mouse brain. Predicted reactivity: Mouse (86%), Rat (82%), Chimpanzee (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RFESD Polyclonal Antibody (Product # PA5-32062). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. Lane D: MCF-7 . 15% SDS PAGE. RFESD Polyclonal Antibody (Product # PA5-32062) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RFESD Polyclonal Antibody (Product # PA5-32062). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 15% SDS PAGE. RFESD Polyclonal Antibody (Product # PA5-32062) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RFESD (Product # PA5-32062) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
