LS-C154242

antibody from LSBio

Targeting: RBL2 p130, Rb2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C154242

Provider

LSBio

Product name

RBL2 / RB2 p130 Antibody LS-C154242

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Spa310 Antibody - Western Blot. Western blot of affinity purified anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kD, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4C. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4C. ECL was used for detection. Personal communication, Ang Sun, Sbarro Institute for Cancer Research and Molecular Medicine, Temple University, Philadelphia, PA.