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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX26545 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX26545, RRID:AB_372878
- Product name
- Rb2 / p130 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 ?g per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4¢XC. Secondary antibody: IRDye800? rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4¢XC. Predicted/Observed size: 130 kDa for p130/Rb2.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.? The staining shows the location of pRb2/p130 in developing mouse tissue.? Other detection systems should yield similar results. Sections were cut at 5-7 ?m, mounted on glass and dried overnight at 37¢XC. ?All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). ?PBS was used for all subsequent washes and for antiserum dilution. ?Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. ?Slides were incubated at 20¢X C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. ?Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20¢X C.? Diaminobenzidine was used as the final chromogen. ?Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
