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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319243 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal pRb2 p130 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal pRb2 p130 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RBL2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.39 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4?. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4?. ECL was used for detection.
- Validation comment
- WB
