R1183

antibody from Acris Antibodies GmbH

Targeting: RBL2 p130, Rb2

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1183

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1183, RRID:AB_1006949

Product name

anti RBL2 / p130

Antibody type

Polyclonal

Antigen

Rb2 (p130) peptide corresponding to the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.1 ml

Concentration

85 mg/ml (by Refractometry)

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure. Immunohistochemical staining of Mouse tissue using R1183 p130 antibody antiserum. The staining shows the location of pRb2/p130 in developing Mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections were deparaffinized in Xylene, rehydrated through a graded Alcohol series and washed in PBS. PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% Hydrogen peroxide and blocked with diluted 10% Normal Goat anti-Rabbit serum. Slides were incubated at 20°C for 1 h with Rabbit anti-pRb2/p130 antibody (1/500) dilution, washed, and then reacted with diluted Biotin conjugated Goat anti-Rabbit antibody for 30 min. Slides were then reacted with Streptavidin-Peroxidase conjugate for 30 min at 20°C. Diaminobenzidine was used as the final chromogen. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.