Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R1183 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1183, RRID:AB_1006949
- Product name
- anti RBL2 / p130
- Antibody type
- Polyclonal
- Antigen
- Rb2 (p130) peptide corresponding to the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 85 mg/ml (by Refractometry)
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure. Immunohistochemical staining of Mouse tissue using R1183 p130 antibody antiserum. The staining shows the location of pRb2/p130 in developing Mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C. All sections were deparaffinized in Xylene, rehydrated through a graded Alcohol series and washed in PBS. PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% Hydrogen peroxide and blocked with diluted 10% Normal Goat anti-Rabbit serum. Slides were incubated at 20°C for 1 h with Rabbit anti-pRb2/p130 antibody (1/500) dilution, washed, and then reacted with diluted Biotin conjugated Goat anti-Rabbit antibody for 30 min. Slides were then reacted with Streptavidin-Peroxidase conjugate for 30 min at 20°C. Diaminobenzidine was used as the final chromogen. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.