ABIN401458

antibody from antibodies-online

Targeting: RBL2 p130, Rb2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Antibody Data

Product number

ABIN401458

Provider

antibodies-online

Product name

anti-Retinoblastoma-Like 2 (p130) (RBL2) antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residueof cysteine was added to facilitate coupling

Reactivity

Human, Rat, Bovine, Canine, Horse

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

1.39 mg/ml (by UV absorbance at 280 nm)

Storage

Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volumedilute 1: 10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mixthoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use thisintermediate dilution when calculating final dilutions as recommended below. Shelf life: 3 month from despatch.

References

Submitted references

A small molecule based on the pRb2/p130 spacer domain leads to inhibition of cdk2 activity, cell cycle arrest and tumor growth reduction in vivo.

Bagella L, Sun A, Tonini T, Abbadessa G, Cottone G, Paggi MG, De Luca A, Claudio PP, Giordano A
Oncogene 2007 Mar 22;26(13):1829-39

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From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway.

Sun A, Bagella L, Tutton S, Romano G, Giordano A
Journal of cellular biochemistry 2007 Dec 15;102(6):1400-4

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Frequent loss of pRb2/p130 in human ovarian carcinoma.

D'Andrilli G, Masciullo V, Bagella L, Tonini T, Minimo C, Zannoni GF, Giuntoli RL 2nd, Carlson JA Jr, Soprano DR, Soprano KJ, Scambia G, Giordano A
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 May 1;10(9):3098-103