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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27433 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PABPN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, mouse brain.
- Concentration
- 0.49 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PABPN1 Polyclonal Antibody (Product # PA5-27433). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. PABPN1 Polyclonal Antibody (Product # PA5-27433) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PABPN1 Polyclonal Antibody (Product # PA5-27433). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 12% SDS PAGE. PABPN1 Polyclonal Antibody (Product # PA5-27433) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PABPN1 Polyclonal Antibody detects PABPN1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PABPN1 protein stained by PABPN1 Polyclonal Antibody (Product # PA5-27433) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PABPN1 (Product # PA5-27433) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
