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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [3]
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- Product number
- PA5-90755 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK5RAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQGLKEVTLL GQNVNSFRDN SEVQFNSAVP TNLSRGFTTN YKTKQGGLRF AHLLDQVSRV; Positive Samples: A-549, Mouse thymus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.73 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CDK5RAP1 Polyclonal antibody (Product # PA5-90755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDK5RAP1 in extracts of various cell lines using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using CDK5RAP1 Polyclonal antibody (Product # PA5-90755) at dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CDK5RAP1 was performed in HeLa cells using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CDK5RAP1 was performed in HeLa cells using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CDK5RAP1 was performed in L-929 cells using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded rat liver using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded human colon carcinoma using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded mouse kidney using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755) at a dilution of 1:100.
