MA5-18288

antibody from Invitrogen Antibodies

Targeting: SLC16A1 MCT, MCT1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-18288

Provider

Invitrogen Antibodies

Product name

MCT1 Monoclonal Antibody (P14612)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

P14612

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Using the Cancer Dependency Map to Identify the Mechanism of Action of a Cytotoxic Alkenyl Derivative from the Fruit of Choerospondias axillaris.

Kil YS, Risinger AL, Petersen CL, Liang H, Grkovic T, O'Keefe BR, Mooberry SL, Cichewicz RH
Journal of natural products 2020 Mar 27;83(3):584-592

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Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.

Lee SH, Hadipour-Lakmehsari S, Kim DH, Di Paola M, Kuzmanov U, Shah S, Lee JJ, Kislinger T, Sharma P, Oudit GY, Gramolini AO
Scientific data 2020 Dec 1;7(1):425

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Interfering cellular lactate homeostasis overcomes Taxol resistance of breast cancer cells through the microRNA-124-mediated lactate transporter (MCT1) inhibition.

Hou L, Zhao Y, Song GQ, Ma YH, Jin XH, Jin SL, Fang YH, Chen YC
Cancer cell international 2019;19:193

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCT1 Monoclonal Antibody (P14612) detects MCT1 protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCT1 protein stained by MCT1 Monoclonal Antibody (P14612) (Product # MA5-18288) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCT1 Monoclonal Antibody (P14612) detects MCT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human colon. MCT1 stained by MCT1 Monoclonal Antibody (P14612) (Product # MA5-18288) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 Co-immunofluorescence analysis demonstrates colocalization of FAM162A and COX20 with known mitochondrial marker, COXIV, and MCT1 colocalization with known plasma membrane protein, Galphai, in isolated adult mouse cardiomyocytes. ( a ) Immunofluorescence analysis of FAM162A (green) co-stained with mitochondrial protein, COXIV (red) in acutely isolated adult mouse cardiomyocytes. Three-dimensional reconstructive analysis demonstrates regions of colocalization (yellow) with a Pearson's coefficient p > 0.5. Scale, 10 mum. ( b ) Immunofluorescence analysis of MCT1 (green) co-stained with known plasma membrane protein, Galphai (red) in acutely isolated adult mouse cardiomyocytes. Three-dimensional reconstructive analysis demonstrates regions of colocalization (yellow) with a Pearson's coefficient p > 0.5. Scale, 10mum. ( c ) Immunofluorescence analysis of COX20 (green) co-stained with mitochondrial protein, COXIV (red) in acutely isolated adult mouse cardiomyocytes. Three-dimensional reconstructive analysis demonstrates regions of colocalization (yellow) with a Pearson's coefficient p > 0.5. Scale, 10mum. Nuclear staining was visualized with Hoechst staining (blue). All images shown are representative of approximately 30-40 total images captured per condition, n = 3 independent biological replicates. All original uncropped microscopy images were uploaded to figshare (10.6084/m9.figshare.11844972.v12).