22787-1-AP

antibody from Proteintech Group

Targeting: SLC16A3 MCT3, MCT4

Provider product page for 22787-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [69]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 22787-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#22787-1-AP, RRID:AB_11182479
Product name: MCT4 antibody
Antibody type: Polyclonal
Description: KD/KO validated MCT4 antibody (Cat. #22787-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
Reactivity: Human, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SLC16A3 protein structure - 22787-1-AP shown in red.

Submitted references Monocarboxylate Transporters 1 and 2 Are Responsible for L-Lactate Uptake in Differentiated Human Neuroblastoma SH-SY5Y Cells.

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Serine synthesis pathway upregulated by E-cadherin is essential for the proliferation and metastasis of breast cancers.

Lee G, Wong C, Cho A, West JJ, Crawford AJ, Russo GC, Si BR, Kim J, Hoffner L, Jang C, Jung M, Leone RD, Konstantopoulos K, Ewald AJ, Wirtz D, Jeong S
bioRxiv : the preprint server for biology 2024 Mar 26;

MCT4-dependent lactate transport: a novel mechanism for cardiac energy metabolism injury and inflammation in type 2 diabetes mellitus.

Ma XM, Geng K, Wang P, Jiang Z, Law BY, Xu Y
Cardiovascular diabetology 2024 Mar 14;23(1):96

Real-time monitoring of glucose metabolism and effects of metformin on HepG2 cells using (13)C in-cell NMR spectroscopy.

Teng M, Li Z, Gu Y, Fan Y, Wang D, Liu M, Li Y, Wei G, Huang Y
Biochemical and biophysical research communications 2024 Jan 29;694:149383

Exercise enhances placental labyrinth trophoblast development by activation of PGC-1α and FNDC5/irisin†.

Chae SA, Du M, Zhu MJ, Son JS
Biology of reproduction 2024 Feb 10;110(2):355-364

Monocarboxylate transporter 4 deficiency enhances high-intensity interval training-induced metabolic adaptations in skeletal muscle.

Tamura Y, Jee E, Kouzaki K, Kotani T, Nakazato K
The Journal of physiology 2024 Apr;602(7):1313-1340

The SIX1/LDHA Axis Promotes Lactate Accumulation and Leads to NK Cell Dysfunction in Pancreatic Cancer.

Ge W, Meng L, Cao S, Hou C, Zhu X, Huang D, Li Q, Peng Y, Jiang K
Journal of immunology research 2023;2023:6891636

Loss of Lactate/Proton Monocarboxylate Transporter 4 Induces Ferroptosis via the AMPK/ACC Pathway and Inhibition of Autophagy on Human Bladder Cancer 5637 Cell Line.

Dong S, Zheng L, Jiang T
Journal of oncology 2023;2023:2830306

Acidification of intracellular pH in MM tumor cells overcomes resistance to hypoxia-mediated apoptosis in vitro and in vivo.

Gastelum G, Kraut J, Veena M, Baibussinov A, Lamb C, Lyons K, Chang EY, Frost P
Frontiers in oncology 2023;13:1268421

Oral administration of procyanidin B2 3,3"-di-O-gallate ameliorates experimental autoimmune encephalomyelitis through immunosuppressive effects on CD4(+) T cells by regulating glycolysis.

Endo K, Sawa T, Tanaka Y, Saiki T, Haga H, Rizeq L, Aso H, Isono M, Niitsuma F, Masaki A, Makabe H, Tanaka S
European journal of pharmacology 2023 Sep 5;954:175879

Icariin-Curcumol promotes docetaxel sensitivity in prostate cancer through modulation of the PI3K-Akt signaling pathway and the Warburg effect.

Xu W, Ding J, Kuang S, Li B, Sun T, Zhu C, Liu J, Zhu L, Li Y, Sheng W
Cancer cell international 2023 Sep 2;23(1):190

Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A.

Zhao X, Stein KR, Chen V, Griffin ME, Lairson LL, Hang HC
Nature chemical biology 2023 Oct;19(10):1205-1214

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle.

She X, Wu Q, Rao Z, Song D, Huang C, Feng S, Liu A, Liu L, Wan K, Li X, Yu C, Qiu C, Luo X, Hu J, Wang G, Xu F, Sun L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Oct;10(28):e2301871

Post-ischemic inactivation of HIF prolyl hydroxylases in endothelium promotes maladaptive kidney repair by inducing glycolysis.

Tiwari R, Sharma R, Rajendran G, Borkowski GS, An SY, Schonfeld M, O'Sullivan J, Schipma MJ, Zhou Y, Courbon G, David V, Quaggin SE, Thorp E, Chandel NS, Kapitsinou PP
bioRxiv : the preprint server for biology 2023 Oct 3;

Evodiamine impairs HIF1A histone lactylation to inhibit Sema3A-mediated angiogenesis and PD-L1 by inducing ferroptosis in prostate cancer.

Yu Y, Huang X, Liang C, Zhang P
European journal of pharmacology 2023 Oct 15;957:176007

SLC16a6, mTORC1, and Autophagy Regulate Ketone Body Excretion in the Intestinal Cells.

Uebanso T, Fukui M, Naito C, Shimohata T, Mawatari K, Takahashi A
Biology 2023 Nov 26;12(12)

NPAS2 promotes aerobic glycolysis and tumor growth in prostate cancer through HIF-1A signaling.

Ma S, Chen Y, Quan P, Zhang J, Han S, Wang G, Qi R, Zhang X, Wang F, Yuan J, Yang X, Jia W, Qin W
BMC cancer 2023 Mar 28;23(1):280

An independent regulator of global release pathways in astrocytes generates a subtype of extracellular vesicles required for postsynaptic function.

Levy-Myers R, Daudelin D, Na CH, Sockanathan S
Science advances 2023 Jun 23;9(25):eadg2067

Nicotine activates HIF-1α and regulates acid extruders through the nicotinic acetylcholine receptor to promote the Warburg effect in non-small cell lung cancer cells.

Huang HK, Lin PC, Huang TT, Hung HY, Huang TW, Huang EY
European journal of pharmacology 2023 Jul 5;950:175778

Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells.

Han S, Zhang H, Liu X, Wen F, Li B, Bie Z, Qiu H, Hu J
Ecotoxicology and environmental safety 2023 Jul 1;259:115063

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.

Yang Z, Yan C, Ma J, Peng P, Ren X, Cai S, Shen X, Wu Y, Zhang S, Wang X, Qiu S, Zhou J, Fan J, Huang H, Gao Q
Nature metabolism 2023 Jan;5(1):61-79

Lactate metabolism is essential in early-onset mitochondrial myopathy.

Chen Z, Bordieanu B, Kesavan R, Lesner NP, Venigalla SSK, Shelton SD, DeBerardinis RJ, Mishra P
Science advances 2023 Jan 4;9(1):eadd3216

Monocarboxylate transporter 4 involves in energy metabolism and drug sensitivity in hypoxia.

Yamaguchi A, Mukai Y, Sakuma T, Narumi K, Furugen A, Yamada Y, Kobayashi M
Scientific reports 2023 Jan 27;13(1):1501

Immunohistochemical evaluation and prognostic value of monocarboxylate transporter 1 (MCT1) and 4 (MCT4) in T-cell non-Hodgkin lymphoma.

Zhao H, Chen Y, Liao YP, Chen HM, Yang QH, Xiao Y, Luo J, Chen ZZ, Yi L, Hu GY
Clinical and experimental medicine 2023 Feb;23(1):55-64

Functional heterogeneity of MCT1 and MCT4 in metabolic reprogramming affects osteosarcoma growth and metastasis.

Sheng G, Gao Y, Wu H, Liu Y, Yang Y
Journal of orthopaedic surgery and research 2023 Feb 22;18(1):131

Fibroblast growth factor 21 protects the liver from apoptosis in a type 1 diabetes mouse model via regulating L-lactate homeostasis.

Yan J, Xie J, Xu S, Guo Y, Ji K, Li C, Gao H, Zhao L
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Dec;168:115737

Bacterial butyrate mediates the anti-atherosclerotic effect of silybin.

Shen HR, Wang ZY, Shen Z, Liu TT, Guo YD, Gao TL, Guo HH, Han YX, Jiang JD
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Dec 31;169:115916

Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p.

Zhong J, Xu A, Xu P, Su M, Wang P, Liu Z, Li B, Liu C, Jiang N
Cell death discovery 2023 Aug 2;9(1):283

Shoutai Wan regulates glycolysis imbalance at the maternal-fetal interface in threatened abortion mice.

Zhang L, Wang S, Ma Y, Song Y, Li D, Liang X, Hao Y, Jiang M, Lv J, Du H
Journal of ethnopharmacology 2023 Aug 10;312:116502

Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion.

Liang X, Tang S, Li D, Song Y, He M, Duan Y, Du H
Evidence-based complementary and alternative medicine : eCAM 2022;2022:8251503

Trophoblast-derived Lactic Acid Orchestrates Decidual Macrophage Differentiation via SRC/LDHA Signaling in Early Pregnancy.

Gao L, Xu QH, Ma LN, Luo J, Muyayalo KP, Wang LL, Huang DH, Xiao XJ, Cheng SB, Mor G, Liao AH
International journal of biological sciences 2022;18(2):599-616

Effects of Fibroblast Growth Factor 21 on Lactate Uptake and Usage in Mice with Diabetes-Associated Cognitive Decline.

Zhao L, Jiang H, Xie J, Shen D, Yi Q, Yan J, Li C, Zheng H, Gao H
Molecular neurobiology 2022 Sep;59(9):5656-5672

Simultaneous targeting of glycolysis and oxidative phosphorylation as a therapeutic strategy to treat diffuse large B-cell lymphoma.

Noble RA, Thomas H, Zhao Y, Herendi L, Howarth R, Dragoni I, Keun HC, Vellano CP, Marszalek JR, Wedge SR
British journal of cancer 2022 Sep;127(5):937-947

Involvement of SLC16A1/MCT1 and SLC16A3/MCT4 in l-lactate transport in the hepatocellular carcinoma cell line.

Mukai Y, Yamaguchi A, Sakuma T, Nadai T, Furugen A, Narumi K, Kobayashi M
Biopharmaceutics & drug disposition 2022 Oct;43(5):183-191

Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat.

Liu Z, Zhang L, Qian C, Zhou Y, Yu Q, Yuan J, Lv Y, Zhang L, Chang X, Li Y, Liu Y
Metabolism: clinical and experimental 2022 Nov;136:155310

Heat shock protein A12A activates migration of hepatocellular carcinoma cells in a monocarboxylate transporter 4-dependent manner.

Min X, Cheng H, Cao X, Chen Z, Zhang X, Li Y, Mao Q, Xue B, Fang L, Liu L, Ding Z
Cell stress & chaperones 2022 Jan;27(1):83-95

Epigallocatechin‑3‑gallate hinders metabolic coupling to suppress colorectal cancer malignancy through targeting aerobic glycolysis in cancer‑associated fibroblasts.

Chen S, Nishi M, Morine Y, Shimada M, Tokunaga T, Kashihara H, Takasu C, Yamada S, Wada Y
International journal of oncology 2022 Feb;60(2)

Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer.

Cappellesso F, Orban MP, Shirgaonkar N, Berardi E, Serneels J, Neveu MA, Di Molfetta D, Piccapane F, Caroppo R, Debellis L, Ostyn T, Joudiou N, Mignion L, Richiardone E, Jordan BF, Gallez B, Corbet C, Roskams T, DasGupta R, Tejpar S, Di Matteo M, Taverna D, Reshkin SJ, Topal B, Virga F, Mazzone M
Nature cancer 2022 Dec;3(12):1464-1483

1,25(OH)(2)D(3) alleviates LPS-induced preeclampsia-like rats impairment in the protective effect by TLR4/NF-kB pathway.

Ma Y, Yang Y, Lv M, Zhang Y, He Q, Zhang Y, Su H, Deng X, Qian Y
Placenta 2022 Dec;130:34-41

Limosilactobacillus reuteri and caffeoylquinic acid synergistically promote adipose browning and ameliorate obesity-associated disorders.

Liu Y, Zhong X, Lin S, Xu H, Liang X, Wang Y, Xu J, Wang K, Guo X, Wang J, Yu M, Li C, Xie C
Microbiome 2022 Dec 15;10(1):226

Nonylphenol inhibited HIF-1alpha regulated aerobic glycolysis and induced ROS mediated apoptosis in rat Sertoli cells.

Jiang Q, Di Q, Shan D, Xu Q
Ecotoxicology and environmental safety 2022 Aug;241:113822

Metformin alleviates the cognitive impairment caused by aluminum by improving energy metabolism disorders in mice.

Song Y, Liu Z, Zhu X, Hao C, Hao W, Wu S, Yang J, Lu X, Jin C
Biochemical pharmacology 2022 Aug;202:115140

Glycometabolic reprogramming-mediated proangiogenic phenotype enhancement of cancer-associated fibroblasts in oral squamous cell carcinoma: role of PGC-1α/PFKFB3 axis.

Li X, Jiang E, Zhao H, Chen Y, Xu Y, Feng C, Li J, Shang Z
British journal of cancer 2022 Aug;127(3):449-461

Anagliptin promotes apoptosis in mouse colon carcinoma cells via MCT-4/lactate-mediated intracellular acidosis.

Li Q, Qin X, Kou X, Li J, Li Z, Chen C
Experimental and therapeutic medicine 2022 Apr;23(4):282

Hypoxia Is a Dominant Remodeler of the Effector T Cell Surface Proteome Relative to Activation and Regulatory T Cell Suppression.

Byrnes JR, Weeks AM, Shifrut E, Carnevale J, Kirkemo L, Ashworth A, Marson A, Wells JA
Molecular & cellular proteomics : MCP 2022 Apr;21(4):100217

The lactate sensor GPR81 regulates glycolysis and tumor growth of breast cancer.

Ishihara S, Hata K, Hirose K, Okui T, Toyosawa S, Uzawa N, Nishimura R, Yoneda T
Scientific reports 2022 Apr 15;12(1):6261

Combination of Exercise and Intake of Amino Acid Mixture Synergistically Induces Beige Adipocyte Formation in Mice.

Kojima T, Esaki N, Tsuda T
Journal of nutritional science and vitaminology 2021;67(4):225-233

Pharmacological Inhibition of MCT4 Reduces 4-Hydroxytamoxifen Sensitivity by Increasing HIF-1α Protein Expression in ER-Positive MCF-7 Breast Cancer Cells.

Nadai T, Narumi K, Furugen A, Saito Y, Iseki K, Kobayashi M
Biological & pharmaceutical bulletin 2021;44(9):1247-1253

Activation of Hippocampal IR/IRS-1 Signaling Contributes to the Treatment with Zuogui Jiangtang Jieyu Decoction on the Diabetes-Related Depression.

Yang H, Ling J, Meng P, Liu J, Lin X, Li W, Wang Y
Evidence-based complementary and alternative medicine : eCAM 2021;2021:6688723

Comprehensive Analysis of the Relationship Between Metabolic Reprogramming and Immune Function in Prostate Cancer.

Xie W, Guo H, Zhang J, Hu L, Wu Y, Wang X
OncoTargets and therapy 2021;14:3251-3266

Elevated Lactate by High-Intensity Interval Training Regulates the Hippocampal BDNF Expression and the Mitochondrial Quality Control System.

Hu J, Cai M, Shang Q, Li Z, Feng Y, Liu B, Xue X, Lou S
Frontiers in physiology 2021;12:629914

Activated Pancreatic Stellate Cells Enhance the Warburg Effect to Cause the Malignant Development in Chronic Pancreatitis.

Tao Y, Shao F, Cai M, Liu Z, Peng Y, Huang Q, Meng F
Frontiers in oncology 2021;11:714598

Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mouse.

Tamura Y, Jee E, Kouzaki K, Kotani T, Nakazato K
Physiological reports 2021 Sep;9(17):e15014

Basigin deficiency prevents anaplerosis and ameliorates insulin resistance and hepatosteatosis.

Ryuge A, Kosugi T, Maeda K, Banno R, Gou Y, Zaitsu K, Ito T, Sato Y, Hirayama A, Tsubota S, Honda T, Nakajima K, Ozaki T, Kondoh K, Takahashi K, Kato N, Ishimoto T, Soga T, Nakagawa T, Koike T, Arima H, Yuzawa Y, Minokoshi Y, Maruyama S, Kadomatsu K
JCI insight 2021 Oct 22;6(20)

In vitro angiogenesis inhibition with selective compounds targeting the key glycolytic enzyme PFKFB3.

Abdali A, Baci D, Damiani I, Belloni F, De Dominicis C, Gelmi ML, Corsini A, Bellosta S
Pharmacological research 2021 Jun;168:105592

Monocarboxylate transporter antagonism reveals metabolic vulnerabilities of viral-driven lymphomas.

Bonglack EN, Messinger JE, Cable JM, Ch'ng J, Parnell KM, Reinoso-Vizcaíno NM, Barry AP, Russell VS, Dave SS, Christofk HR, Luftig MA
Proceedings of the National Academy of Sciences of the United States of America 2021 Jun 22;118(25)

Lactate sensing mechanisms in arterial chemoreceptor cells.

Torres-Torrelo H, Ortega-Sáenz P, Gao L, López-Barneo J
Nature communications 2021 Jul 6;12(1):4166

Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non-carcinogenic L929 fibroblast cells.

Li X, Zhou X, Liu Y, Fan J, Huo H, Yao J, Wang L, Ma N
Oncology letters 2021 Jan;21(1):44

Glycolysis is suppressed by DCZ0801-induced inactivation of the Akt/mTOR pathway in Multiple Myeloma.

Feng Q, Yao Q, Li B, Xie Y, Zhang H, Xu Z, Lu K, Hu K, Cheng Y, Shi B, Huang C, Li L, Wu X, You S, Shi J, Zhu W
Journal of Cancer 2020;11(16):4907-4916

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Zhang X, Dong Y, Zhao M, Ding L, Yang X, Jing Y, Song Y, Chen S, Hu Q, Ni Y
Theranostics 2020;10(26):12044-12059

Identification of the essential extracellular aspartic acids conserved in human monocarboxylate transporters 1, 2, and 4.

Yamaguchi A, Narumi K, Furugen A, Iseki K, Kobayashi M
Biochemical and biophysical research communications 2020 Sep 3;529(4):1061-1065

Metabolic reprogramming of normal oral fibroblasts correlated with increased glycolytic metabolism of oral squamous cell carcinoma and precedes their activation into carcinoma associated fibroblasts.

Zhang Z, Gao Z, Rajthala S, Sapkota D, Dongre H, Parajuli H, Suliman S, Das R, Li L, Bindoff LA, Costea DE, Liang X
Cellular and molecular life sciences : CMLS 2020 Mar;77(6):1115-1133

Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature.

Jiang Z, Xiong H, Yang S, Lu Y, Deng Y, Yao J, Yao J
Advanced healthcare materials 2020 Jun;9(12):e2000075

Breaking Glucose Transporter 1/Pyruvate Kinase M2 Glycolytic Loop Is Required for Cantharidin Inhibition of Metastasis in Highly Metastatic Breast Cancer.

Pan Y, Zheng Q, Ni W, Wei Z, Yu S, Jia Q, Wang M, Wang A, Chen W, Lu Y
Frontiers in pharmacology 2019;10:590

Acidic Stress Triggers Sodium-Coupled Bicarbonate Transport and Promotes Survival in A375 Human Melanoma Cells.

Yang OCY, Loh SH
Scientific reports 2019 May 2;9(1):6858

Homology modeling and site-directed mutagenesis identify amino acid residues underlying the substrate selection mechanism of human monocarboxylate transporters 1 (hMCT1) and 4 (hMCT4).

Futagi Y, Kobayashi M, Narumi K, Furugen A, Iseki K
Cellular and molecular life sciences : CMLS 2019 Dec;76(24):4905-4921

Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.

Jiang E, Xu Z, Wang M, Yan T, Huang C, Zhou X, Liu Q, Wang L, Chen Y, Wang H, Liu K, Shao Z, Shang Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Apr;33(4):5690-5703

Prognostic value of monocarboxylate transporter 4 in patients with esophageal squamous cell carcinoma.

Cheng B, Chen X, Li Y, Huang X, Yu J
Oncology reports 2018 Nov;40(5):2906-2915

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.

Shi H, Jiang H, Wang L, Cao Y, Liu P, Xu X, Wang Y, Sun L, Niu H
Cell cycle (Georgetown, Tex.) 2015;14(19):3058-65

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 22787-1-AP(SLC16A3 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The SLC16A3 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human SLC16A3. This antibody recognizes human antigen. The SLC16A3 antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.
Appendix: 22787-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 22787-1-AP(SLC16A3 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 22787-1-AP(SLC16A3 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue