Antibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 13-0037-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD3 Monoclonal Antibody (OKT3), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- OKT3
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence.
TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions.
MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.
Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.
Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells.
Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4+ T Cell Immunity.
Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.
"Waltz" of Cell Membrane-Coated Nanoparticles on Lipid Bilayers: Tracking Single Particle Rotation in Ligand-Receptor Binding.
Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.
Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse.
Astrocytic tight junctions control inflammatory CNS lesion pathogenesis.
CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b+ T cell lymphoma.
Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.
Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.
T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.
Obesity decreases B cell responses in young and elderly individuals.
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.
F-actin flow drives affinity maturation and spatial organization of LFA-1 at the immunological synapse.
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.
Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.
N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.
Ligand mobility modulates immunological synapse formation and T cell activation.
HIV envelope gp120 activates LFA-1 on CD4 T-lymphocytes and increases cell susceptibility to LFA-1-targeting leukotoxin (LtxA).
NKG2D receptor regulates human effector T-cell cytokine production.
Swadling L, Pallett LJ, Diniz MO, Baker JM, Amin OE, Stegmann KA, Burton AR, Schmidt NM, Jeffery-Smith A, Zakeri N, Suveizdyte K, Froghi F, Fusai G, Rosenberg WM, Davidson BR, Schurich A, Simon AK, Maini MK
Cell reports 2020 Jan 21;30(3):687-698.e6
Cell reports 2020 Jan 21;30(3):687-698.e6
TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions.
Leng T, Akther HD, Hackstein CP, Powell K, King T, Friedrich M, Christoforidou Z, McCuaig S, Neyazi M, Arancibia-Cárcamo CV, Hagel J, Powrie F, Oxford IBD Investigators., Peres RS, Millar V, Ebner D, Lamichhane R, Ussher J, Hinks TSC, Marchi E, Willberg C, Klenerman P
Cell reports 2019 Sep 17;28(12):3077-3091.e5
Cell reports 2019 Sep 17;28(12):3077-3091.e5
MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.
Lv M, Li Z, Liu J, Lin F, Zhang Q, Li Z, Wang Y, Wang K, Xu Y
Molecular medicine reports 2019 Oct;20(4):3617-3624
Molecular medicine reports 2019 Oct;20(4):3617-3624
Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.
Rossatti P, Ziegler L, Schregle R, Betzler VM, Ecker M, Rossy J
Cells 2019 Nov 4;8(11)
Cells 2019 Nov 4;8(11)
Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells.
Levring TB, Kongsbak-Wismann M, Rode AKO, Al-Jaberi FAH, Lopez DV, Met Ö, Woetmann A, Bonefeld CM, Ødum N, Geisler C
Scientific reports 2019 Nov 13;9(1):16725
Scientific reports 2019 Nov 13;9(1):16725
Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4+ T Cell Immunity.
Binnewies M, Mujal AM, Pollack JL, Combes AJ, Hardison EA, Barry KC, Tsui J, Ruhland MK, Kersten K, Abushawish MA, Spasic M, Giurintano JP, Chan V, Daud AI, Ha P, Ye CJ, Roberts EW, Krummel MF
Cell 2019 Apr 18;177(3):556-571.e16
Cell 2019 Apr 18;177(3):556-571.e16
Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.
Pan H, Liu J, Deng W, Xing J, Li Q, Wang Z
International journal of nanomedicine 2018;13:3189-3201
International journal of nanomedicine 2018;13:3189-3201
"Waltz" of Cell Membrane-Coated Nanoparticles on Lipid Bilayers: Tracking Single Particle Rotation in Ligand-Receptor Binding.
Yu Y, Gao Y, Yu Y
ACS nano 2018 Dec 26;12(12):11871-11880
ACS nano 2018 Dec 26;12(12):11871-11880
Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.
Geißler K, Markwart R, Requardt RP, Weigel C, Schubert K, Scherag A, Rubio I, Guntinas-Lichius O
PloS one 2017;12(9):e0183214
PloS one 2017;12(9):e0183214
Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.
Hernández Del Pino RE, Pellegrini JM, Rovetta AI, Peña D, Álvarez GI, Rolandelli A, Musella RM, Palmero DJ, Malbran A, Pasquinelli V, García VE
Immunology and cell biology 2017 Sep;95(8):716-728
Immunology and cell biology 2017 Sep;95(8):716-728
Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.
Lim AI, Li Y, Lopez-Lastra S, Stadhouders R, Paul F, Casrouge A, Serafini N, Puel A, Bustamante J, Surace L, Masse-Ranson G, David E, Strick-Marchand H, Le Bourhis L, Cocchi R, Topazio D, Graziano P, Muscarella LA, Rogge L, Norel X, Sallenave JM, Allez M, Graf T, Hendriks RW, Casanova JL, Amit I, Yssel H, Di Santo JP
Cell 2017 Mar 9;168(6):1086-1100.e10
Cell 2017 Mar 9;168(6):1086-1100.e10
Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse.
Tello-Lafoz M, Martínez-Martínez G, Rodríguez-Rodríguez C, Albar JP, Huse M, Gharbi S, Merida I
Traffic (Copenhagen, Denmark) 2017 Aug;18(8):491-504
Traffic (Copenhagen, Denmark) 2017 Aug;18(8):491-504
Astrocytic tight junctions control inflammatory CNS lesion pathogenesis.
Horng S, Therattil A, Moyon S, Gordon A, Kim K, Argaw AT, Hara Y, Mariani JN, Sawai S, Flodby P, Crandall ED, Borok Z, Sofroniew MV, Chapouly C, John GR
The Journal of clinical investigation 2017 Aug 1;127(8):3136-3151
The Journal of clinical investigation 2017 Aug 1;127(8):3136-3151
CD16+ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.
Zhu H, Hu F, Sun X, Zhang X, Zhu L, Liu X, Li X, Xu L, Shi L, Gan Y, Su Y
Frontiers in immunology 2016;7:512
Frontiers in immunology 2016;7:512
CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b+ T cell lymphoma.
Bagchi S, Li S, Wang CR
Oncoimmunology 2016;5(9):e1213932
Oncoimmunology 2016;5(9):e1213932
Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.
Chen J, Wang Q, Feng X, Zhang Z, Geng L, Xu T, Wang D, Sun L
Stem cells international 2016;2016:4062789
Stem cells international 2016;2016:4062789
Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.
Pageon SV, Tabarin T, Yamamoto Y, Ma Y, Nicovich PR, Bridgeman JS, Cohnen A, Benzing C, Gao Y, Crowther MD, Tungatt K, Dolton G, Sewell AK, Price DA, Acuto O, Parton RG, Gooding JJ, Rossy J, Rossjohn J, Gaus K
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 13;113(37):E5454-63
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 13;113(37):E5454-63
T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.
Guillou L, Babataheri A, Saitakis M, Bohineust A, Dogniaux S, Hivroz C, Barakat AI, Husson J
Molecular biology of the cell 2016 Nov 7;27(22):3574-3582
Molecular biology of the cell 2016 Nov 7;27(22):3574-3582
Obesity decreases B cell responses in young and elderly individuals.
Frasca D, Ferracci F, Diaz A, Romero M, Lechner S, Blomberg BB
Obesity (Silver Spring, Md.) 2016 Mar;24(3):615-25
Obesity (Silver Spring, Md.) 2016 Mar;24(3):615-25
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
Liu Z, Han H, He X, Li S, Wu C, Yu C, Wang S
Oncology letters 2016 Mar;11(3):1829-1834
Oncology letters 2016 Mar;11(3):1829-1834
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
You Y, Tan JX, Dai HS, Chen HW, Xu XJ, Yang AG, Zhang YJ, Bai LH, Bie P
Oncotarget 2016 Aug 30;7(35):57099-57116
Oncotarget 2016 Aug 30;7(35):57099-57116
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Kim SY, Theunissen JW, Balibalos J, Liao-Chan S, Babcock MC, Wong T, Cairns B, Gonzalez D, van der Horst EH, Perez M, Levashova Z, Chinn L, D'Alessio JA, Flory M, Bermudez A, Jackson DY, Ha E, Monteon J, Bruhns MF, Chen G, Migone TS
Blood cancer journal 2015 May 29;5:e316
Blood cancer journal 2015 May 29;5:e316
Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.
Kumari S, Depoil D, Martinelli R, Judokusumo E, Carmona G, Gertler FB, Kam LC, Carman CV, Burkhardt JK, Irvine DJ, Dustin ML
eLife 2015 Mar 11;4
eLife 2015 Mar 11;4
F-actin flow drives affinity maturation and spatial organization of LFA-1 at the immunological synapse.
Comrie WA, Babich A, Burkhardt JK
The Journal of cell biology 2015 Feb 16;208(4):475-91
The Journal of cell biology 2015 Feb 16;208(4):475-91
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
Roberts RA, Eitas TK, Byrne JD, Johnson BM, Short PJ, McKinnon KP, Reisdorf S, Luft JC, DeSimone JM, Ting JP
Biomaterials 2015 Dec;72:1-10
Biomaterials 2015 Dec;72:1-10
TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.
Reichwald K, Jørgensen TZ, Tougaard P, Skov S
PloS one 2014;9(1):e85793
PloS one 2014;9(1):e85793
Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.
Kumaresan PR, Manuri PR, Albert ND, Maiti S, Singh H, Mi T, Roszik J, Rabinovich B, Olivares S, Krishnamurthy J, Zhang L, Najjar AM, Huls MH, Lee DA, Champlin RE, Kontoyiannis DP, Cooper LJ
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10660-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10660-5
N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.
Mellergaard M, Skovbakke SL, Schneider CL, Lauridsen F, Andresen L, Jensen H, Skov S
The Journal of biological chemistry 2014 Jul 18;289(29):20078-91
The Journal of biological chemistry 2014 Jul 18;289(29):20078-91
Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.
Guo YE, Riley KJ, Iwasaki A, Steitz JA
Molecular cell 2014 Apr 10;54(1):67-79
Molecular cell 2014 Apr 10;54(1):67-79
Ligand mobility modulates immunological synapse formation and T cell activation.
Hsu CJ, Hsieh WT, Waldman A, Clarke F, Huseby ES, Burkhardt JK, Baumgart T
PloS one 2012;7(2):e32398
PloS one 2012;7(2):e32398
HIV envelope gp120 activates LFA-1 on CD4 T-lymphocytes and increases cell susceptibility to LFA-1-targeting leukotoxin (LtxA).
Hioe CE, Tuen M, Vasiliver-Shamis G, Alvarez Y, Prins KC, Banerjee S, Nádas A, Cho MW, Dustin ML, Kachlany SC
PloS one 2011;6(8):e23202
PloS one 2011;6(8):e23202
NKG2D receptor regulates human effector T-cell cytokine production.
Barber A, Sentman CL
Blood 2011 Jun 16;117(24):6571-81
Blood 2011 Jun 16;117(24):6571-81
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 605 and 0.03 µg of Biotin Mouse IgG2a K Isotype Control (Product # 13-4724-85) (left) or 0.03 µg of Anti-Human CD3 FITC (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
- Conjugate
- Biotin