Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 25-0037-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD3 Monoclonal Antibody (OKT3), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OKT3
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.
Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.
Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.
Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.
Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.
Pan H, Liu J, Deng W, Xing J, Li Q, Wang Z
International journal of nanomedicine 2018;13:3189-3201
International journal of nanomedicine 2018;13:3189-3201
Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.
Hernández Del Pino RE, Pellegrini JM, Rovetta AI, Peña D, Álvarez GI, Rolandelli A, Musella RM, Palmero DJ, Malbran A, Pasquinelli V, García VE
Immunology and cell biology 2017 Sep;95(8):716-728
Immunology and cell biology 2017 Sep;95(8):716-728
Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.
Chen J, Wang Q, Feng X, Zhang Z, Geng L, Xu T, Wang D, Sun L
Stem cells international 2016;2016:4062789
Stem cells international 2016;2016:4062789
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.
Liu Z, Han H, He X, Li S, Wu C, Yu C, Wang S
Oncology letters 2016 Mar;11(3):1829-1834
Oncology letters 2016 Mar;11(3):1829-1834
MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.
You Y, Tan JX, Dai HS, Chen HW, Xu XJ, Yang AG, Zhang YJ, Bai LH, Bie P
Oncotarget 2016 Aug 30;7(35):57099-57116
Oncotarget 2016 Aug 30;7(35):57099-57116
A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.
Kim SY, Theunissen JW, Balibalos J, Liao-Chan S, Babcock MC, Wong T, Cairns B, Gonzalez D, van der Horst EH, Perez M, Levashova Z, Chinn L, D'Alessio JA, Flory M, Bermudez A, Jackson DY, Ha E, Monteon J, Bruhns MF, Chen G, Migone TS
Blood cancer journal 2015 May 29;5:e316
Blood cancer journal 2015 May 29;5:e316
Towards programming immune tolerance through geometric manipulation of phosphatidylserine.
Roberts RA, Eitas TK, Byrne JD, Johnson BM, Short PJ, McKinnon KP, Reisdorf S, Luft JC, DeSimone JM, Ting JP
Biomaterials 2015 Dec;72:1-10
Biomaterials 2015 Dec;72:1-10
TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.
Reichwald K, Jørgensen TZ, Tougaard P, Skov S
PloS one 2014;9(1):e85793
PloS one 2014;9(1):e85793
Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.
Kumaresan PR, Manuri PR, Albert ND, Maiti S, Singh H, Mi T, Roszik J, Rabinovich B, Olivares S, Krishnamurthy J, Zhang L, Najjar AM, Huls MH, Lee DA, Champlin RE, Kontoyiannis DP, Cooper LJ
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10660-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 22;111(29):10660-5
Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.
Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW
PLoS pathogens 2012;8(11):e1002984
PLoS pathogens 2012;8(11):e1002984
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or Anti-Human CD3 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.