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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-29387 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3g Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD3g Polyclonal Antibody (Product # PA5-29387). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 12% SDS PAGE. CD3g Polyclonal Antibody (Product # PA5-29387) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CD3g was performed in paraffin-embedded human PANC1 xenograft tissue using CD3g Polyclonal Antibody (Product # PA5-29387) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of CD81 was performed in mouse splenocytes cells fixed in 4% paraformaldehyde at 4°C for 15 min. The primary antibody used was CD81 Polyclonal Antibody (Product # PA5-85150) (red) at a dilution of 1:50. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
