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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MA1-25322 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Coilin Monoclonal Antibody (Pdelta)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa nuclear extract.
- Antibody clone number
- Pdelta
- Concentration
- 1.9 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Coilin Monoclonal Antibody (Pdelta) (Product # MA1-25322) and a band at 63 kDa corresponding to Coilin was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), A-431 (Lane 3), K-562 (Lane 4), SH-SY5Y (Lane 5), Raji (Lane 6), U-2 OS (Lane 7) and MCF7 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2µg/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
