PA5-29531

antibody from Invitrogen Antibodies

Targeting: COIL CLN80, p80-coilin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29531

Provider

Invitrogen Antibodies

Product name

Coilin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2 , HepG2 nuclear extract, 293T nuclear extract. Predicted reactivity: Rhesus Monkey (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.66 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis.

Schmitz K, Cox J, Esser LM, Voss M, Sander K, Löffler A, Hillebrand F, Erkelenz S, Schaal H, Kähne T, Klinker S, Zhang T, Nagel-Steger L, Willbold D, Seggewiß S, Schlütermann D, Stork B, Grimmler M, Wesselborg S, Peter C
Nucleic acids research 2021 Jun 21;49(11):6437-6455

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Coilin using 30 µg of A) 293T (B) A431 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Coilin polyclonal antibody (Product # PA5-29531) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Coilin was performed by separating 30 µg of various whole cell lysates/extracts by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a Coilin Polyclonal Antibody (Product # PA5-29531) at a dilution of 1:1000. (A) 293T and (B) 293T nuclear extract

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Coilin Polyclonal Antibody (Product # PA5-29531). HeLa whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin Polyclonal Antibody (Product # PA5-29531) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Coilin was performed by separating 30 µg of (A) HepG2 and (B) HepG2 nuclear extract by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a Coilin Polyclonal Antibody (Product # PA5-29531) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Coilin in methanol-fixed HeLa cells using a Coilin polyclonal antibody (Product # PA5-29531) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. Decrease of endogenous ULK1 results in a decreased number of Cajal bodies. ( A , B ) HEK293T cells were treated with 50 nM ULK1,2 siRNA or non-targeting control for 48 h. ( A , C ) The cells were fixed and Cajal bodies were visualized with antibody staining against Coilin (green) and SMN (red). The DNA was stained with DAPI (blue). ( B ) Cell lysates of siRNA-treated cells were analyzed by Tris/Glycine-SDS-PAGE using antibodies against ULK1, ULK2, and Tubulin. The downregulation of ULK1 causes a reduction in the snRNP storage pool. In the boxplot diagram, the 'box' represents 25-75% of all values and the mean (red), standard deviation, and out layers are visualized. HEK293T cells show an average of 1.54 (n = 500) Cajal bodies. Treatment of cells with siRNA caused a 1. significant decrease in the number of Cajal bodies. ( C , D ) The phosphorylation status of pICln influences the number of Cajal bodies per cell. ( D ) Overexpression of pICln in Flp-In T-Rex cells causes an increase in the number of Cajal bodies, mean 1.69 (n = 502). Phosphomutants of pICln (S193, 195, 197A, or D), cause a decrease in the number of Cajal bodies per cell. The P -value was calculated with Origin using the Mann-Whitney U test. *** P < 0.005; scale bars: 10 mum (A and C).