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- Antibody Data
- Antigen structure
- References [4]
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- Product number
- AP8074c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP8074c, RRID:AB_2251243
- Product name
- NEK2 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references The centrosomal kinase NEK2 is a novel splicing factor kinase involved in cell survival.
Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation.
Increased expression and nuclear localization of the centrosomal kinase Nek2 in human testicular seminomas.
Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase.
Naro C, Barbagallo F, Chieffi P, Bourgeois CF, Paronetto MP, Sette C
Nucleic acids research 2014 Mar;42(5):3218-27
Nucleic acids research 2014 Mar;42(5):3218-27
Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation.
van Ree JH, Jeganathan KB, Malureanu L, van Deursen JM
The Journal of cell biology 2010 Jan 11;188(1):83-100
The Journal of cell biology 2010 Jan 11;188(1):83-100
Increased expression and nuclear localization of the centrosomal kinase Nek2 in human testicular seminomas.
Barbagallo F, Paronetto MP, Franco R, Chieffi P, Dolci S, Fry AM, Geremia R, Sette C
The Journal of pathology 2009 Feb;217(3):431-41
The Journal of pathology 2009 Feb;217(3):431-41
Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase.
Wu W, Baxter JE, Wattam SL, Hayward DG, Fardilha M, Knebel A, Ford EM, da Cruz e Silva EF, Fry AM
The Journal of biological chemistry 2007 Sep 7;282(36):26431-40
The Journal of biological chemistry 2007 Sep 7;282(36):26431-40
No comments: Submit comment
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from 293T, Hela, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410)(Cat. #AP8074c). AP8074c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-NEK2 Antibody (Center) (Cat.#AP8074c) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
