LS-B11803

antibody from LSBio

Targeting: NEK2 NEK2A, NLK1, PPP1R111, RP67

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B11803

Provider

LSBio

Product name

IHC-plus™ NEK2 Antibody (aa396-426) LS-B11803

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Mouse

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of lysates from 293T, HeLa, Jurkat, K562, MCF-7, and mouse NIH/3T3 cell line (from left to right), using NEK2 Antibody (C410). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of anti-NEK2 Antibody in HL60 cell line lysates (35 ug/lane). NEK2(arrow) was detected using the purified antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)