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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00004751-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004751-B01P, RRID:AB_1577646
- Product name
- NEK2 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human NEK2 protein.
- Antigen sequence
MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILV
WKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRY
YDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKER
QYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHR
DLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKT
FVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELC
ALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELN
EIITRMLNLKDYHRPSVEEILENPLIADLVADEQR
RNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQE
RERALKAREERLEQKEQELCVRERLAEDKLARAEN
LLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVH
FSGESKENIMRSENSESQLTSKSKCKDLKKRLHAA
QLRAQALSDIEKNYQLKSRQILGMR- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NEK2 expression in transfected 293T cell line (H00004751-T01) by NEK2 MaxPab polyclonal antibody.Lane 1: NEK2 transfected lysate(48.95 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NEK2 MaxPab polyclonal antibody. Western Blot analysis of NEK2 expression in rat brain.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to NEK2 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
