H00004751-D01

antibody from Abnova Corporation

Targeting: NEK2 NEK2A, NLK1, PPP1R111, RP67

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004751-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004751-D01, RRID:AB_10629452

Product name

NEK2 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human NEK2 protein.

Antigen sequence

MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILV
WKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRY
YDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKER
QYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHR
DLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKT
FVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELC
ALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELN
EIITRMLNLKDYHRPSVEEILENPLIADLVADEQR
RNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQE
RERALKAREERLEQKEQELCVRERLAEDKLARAEN
LLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVH
FSGESKENIMRSENSESQLTSKSKCKDLKKRLHAA
QLRAQALSDIEKNYQLKSRQILGMR


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NEK2 MaxPab rabbit polyclonal antibody. Western Blot analysis of NEK2 expression in HeLa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NEK2 expression in transfected 293T cell line (H00004751-T02) by NEK2 MaxPab polyclonal antibody.Lane 1: NEK2 transfected lysate(51.80 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of NEK2 transfected lysate using anti-NEK2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NEK2 purified MaxPab mouse polyclonal antibody (B01P) (H00004751-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol