Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00004751-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004751-M01, RRID:AB_829669
- Product name
- Mouse Monoclonal NEK2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NEK2 (2F6)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody (M01), clone 2F6.Lane 1: NEK2 transfected lysate(51.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: NEK2 Antibody (2F6) [H00004751-M01] - Detection limit for recombinant GST tagged NEK2 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of NEK2 transfected lysate using anti-NEK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEK2 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NEK2 Antibody (2F6) [H00004751-M01] - Analysis of monoclonal antibody to NEK2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.