Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN2842150 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (AA 396-426), (Center) antibody
- Antibody type
- Polyclonal
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 396-426, Center
- Antibody clone number
- RB2959
- Vial size
- 80 μL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references The centrosomal kinase NEK2 is a novel splicing factor kinase involved in cell survival.
Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation.
Increased expression and nuclear localization of the centrosomal kinase Nek2 in human testicular seminomas.
Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase.
Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation.
Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation.
Naro C, Barbagallo F, Chieffi P, Bourgeois CF, Paronetto MP, Sette C
Nucleic acids research 2014 Mar;42(5):3218-27
Nucleic acids research 2014 Mar;42(5):3218-27
Overexpression of the E2 ubiquitin-conjugating enzyme UbcH10 causes chromosome missegregation and tumor formation.
van Ree JH, Jeganathan KB, Malureanu L, van Deursen JM
The Journal of cell biology 2010 Jan 11;188(1):83-100
The Journal of cell biology 2010 Jan 11;188(1):83-100
Increased expression and nuclear localization of the centrosomal kinase Nek2 in human testicular seminomas.
Barbagallo F, Paronetto MP, Franco R, Chieffi P, Dolci S, Fry AM, Geremia R, Sette C
The Journal of pathology 2009 Feb;217(3):431-41
The Journal of pathology 2009 Feb;217(3):431-41
Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase.
Wu W, Baxter JE, Wattam SL, Hayward DG, Fardilha M, Knebel A, Ford EM, da Cruz e Silva EF, Fry AM
The Journal of biological chemistry 2007 Sep 7;282(36):26431-40
The Journal of biological chemistry 2007 Sep 7;282(36):26431-40
Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation.
Eto M, Elliott E, Prickett TD, Brautigan DL
The Journal of biological chemistry 2002 Nov 15;277(46):44013-20
The Journal of biological chemistry 2002 Nov 15;277(46):44013-20
Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation.
Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH
The Journal of biological chemistry 2002 Dec 20;277(51):49408-16
The Journal of biological chemistry 2002 Dec 20;277(51):49408-16
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
