200-301-390

antibody from Invitrogen Antibodies

Targeting: KRT6B KRTL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

200-301-390

Provider

Invitrogen Antibodies

Product name

Keratin Monoclonal Antibody (C11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Antibody clone number

C11

Concentration

1.3 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot using ROCKLAND Immunochemicals Mouse Anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximately 100 ng of keratin from human epidermis (Sigma p/n K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence Microscopy of Rockland Immunochemicals Anti-Keratin antibody (200-301-390) was used with Rocklands Dylight 488 goat anti-mouse 610-141-121 (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, Rocklands polyclonal Anti-HDAC-1 antibody (600-401-879) was used with Atto425 Anti-Rabbit IgG 611-151-122 (shown in red) to detect HDAC-1. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence using ROCKLAND Immunochemicals Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 µm where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC conjugated Goat-a-Mouse IgG [H&L] (610-102-121) for detection

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of Mouse anti-Keratin antibody. Tissue: human prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Keratin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Keratin as precipitated red signal with hematoxylin purple nuclear counterstain.