Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-17065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dynactin 4 Monoclonal Antibody (3G9D7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17065 targets DCTN4 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17065 immunogen is purified recombinant fragment of human DCTN4 (amino acids: 57-298) expressed in E. Coli. MA5-17065 detects DCTN4 which has a predicted molecular weight of approximately 52.3kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G9D7
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCTN4 monoclonal antibody (Product # MA5-17065) against human DCTN4 recombinant protein. Expected MW is 53.2 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCTN4 in HEK293 (lane 1) and DCTN4-hIgGFc transfected HEK293 (lane 2) cell lysate using DCTN4 monoclonal antibody (Product # MA5-17065).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCTN4 using DCTN4 monoclonal antibody (Product # MA5-17065) in Raw264.7 (1) and NIH3T3 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCTN4 in Raw264.7 (lane 1) and NIH-3T3 (lane 2) cell lysate using DCTN4 monoclonal antibody (Product # MA5-17065).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCTN4 monoclonal antibody (Product # MA5-17065) against human DCTN4 recombinant protein. Expected MW is 53.2 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCTN4 in HEK293 (lane 1) and DCTN4-hIgGFc transfected HEK293 (lane 2) cell lysate using DCTN4 monoclonal antibody (Product # MA5-17065).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using DCTN4 monoclonal antibody (Product # MA5-17065) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using DCTN4 monoclonal antibody (Product # MA5-17065) (green). Blue: fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DCTN4 monoclonal antibody (Product # MA5-17065) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 monoclonal antibody (Product # MA5-17065) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DCTN4 monoclonal antibody (Product # MA5-17065) with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DCTN4 monoclonal antibody (Product # MA5-17065) with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HEK293 cells using DCTN4 monoclonal antibody (Product # MA5-17065) (green) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 cells using DCTN4 monoclonal antibody (Product # MA5-17065) (green) and negative control (red).