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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB600-480 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-480, RRID:AB_10000877
- Product name
- Rabbit Polyclonal LDB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. LDB2
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.06 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LDB2 Antibody [NB600-480] - Analysis of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LDB2 Antibody [NB600-480] - Used at a 5 ug/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
