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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10273 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10273, RRID:AB_1676511
- Product name
- Ldb2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Ldb2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Ubiquitination-dependent cofactor exchange on LIM homeodomain transcription factors.
Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis.
A family of LIM domain-associated cofactors confer transcriptional synergism between LIM and Otx homeodomain proteins.
Ostendorff HP, Peirano RI, Peters MA, Schlüter A, Bossenz M, Scheffner M, Bach I
Nature 2002 Mar 7;416(6876):99-103
Nature 2002 Mar 7;416(6876):99-103
Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis.
Toyama R, Kobayashi M, Tomita T, Dawid IB
Mechanisms of development 1998 Feb;71(1-2):197-200
Mechanisms of development 1998 Feb;71(1-2):197-200
A family of LIM domain-associated cofactors confer transcriptional synergism between LIM and Otx homeodomain proteins.
Bach I, Carrière C, Ostendorff HP, Andersen B, Rosenfeld MG
Genes & development 1997 Jun 1;11(11):1370-80
Genes & development 1997 Jun 1;11(11):1370-80
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Ldb2 polyclonal antibody (Cat # PAB10273) showsdetection of a 43 KDa band corresponding to Ldb2 in a lysates prepared from human kidney (Lane 1) and mouse spleen (Lane 2) tissues.Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of Ldb2 polyclonal antibody.Detection occurred using a 1 : 5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hourat room temperature.A chemiluminescence system was used for signal detection using a 1 min exposure time.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining with Ldb2 polyclonal antibody (Cat # PAB10273) was used at a 5 ug/mL to detect Ldb2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
