sc-28735

antibody from Santa Cruz Biotechnology

Targeting: CBX5 HP1, HP1-ALPHA, HP1Hs-alpha

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28735

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28735, RRID:AB_2071368

Product name

Anti-CBX5

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Epigenetic changes and suppression of the nuclear factor of activated T cell 1 (NFATC1) promoter in human lymphomas with defects in immunoreceptor signaling.

Akimzhanov A, Krenacs L, Schlegel T, Klein-Hessling S, Bagdi E, Stelkovics E, Kondo E, Chuvpilo S, Wilke P, Avots A, Gattenlöhner S, Müller-Hermelink HK, Palmetshofer A, Serfling E
The American journal of pathology 2008 Jan;172(1):215-24

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Nuclear reprogramming: the zygotic transcription program is established through an “erase-and-rebuild” strategy

Feng Sun, Haiyan Fang, Ruizhen Li, Tianlong Gao, Junke Zheng, Xuejin Chen, Wenqin Ying, Hui Z Sheng
Cell Research 2007 Feb;17(2):117-134

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 22

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human pancreas shows distinct nuclear positivity in islet cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.